Pathogen-Host Interface Laboratory, Department of Cell Biology, The University of Brasilia, Brasilia, DF, Brazil.
Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, The University of Brasilia, Brasilia, DF, Brazil.
Trends Parasitol. 2020 Mar;36(3):250-265. doi: 10.1016/j.pt.2019.12.014. Epub 2020 Jan 29.
Triatomines are blood-feeding insects that prey on vertebrate hosts. Their saliva is largely responsible for their feeding success. The triatomine salivary content has been studied over the past decades, revealing multifunctional bioactive proteins targeting the host´s hemostasis and immune system. Recently, sequencing of salivary-gland mRNA libraries revealed increasingly complex and complete transcript databases that have been used to validate the expression of deduced proteins through proteomics. This review provides an insight into the journey of discovery and characterization of novel molecules in triatomine saliva.
锥蝽是一种以脊椎动物为宿主的吸血昆虫。它们的唾液在很大程度上决定了它们的取食成功。在过去的几十年里，人们对锥蝽的唾液成分进行了研究，揭示了多种针对宿主止血和免疫系统的多功能生物活性蛋白。最近，唾液腺 mRNA 文库的测序揭示了越来越复杂和完整的转录数据库，这些数据库被用于通过蛋白质组学验证推断蛋白的表达。本文综述了在锥蝽唾液中发现和鉴定新型分子的探索历程。
