Carvajal G, Thilly W
Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge 02139.
Plant Foods Hum Nutr. 1988;38(2):105-14. doi: 10.1007/BF01091714.
Cultured AHH1 human lymphoblast cells were exposed to an extract of Mintostachys mollis, which is used as a medicinal plant and preservative in Peru, to detect its mutagenic activity. A three-day exposure to concentrations of 1, 10, 30 and 50 micrograms/ml resulted in induced mutant fraction of 1.02, 1.79, 2.21 and 3.80 mutations per million cells per day, expressed as 6-Thioguanine resistance. A concentration of 10 micrograms/ml induced 11.9 times the spontaneous mutant fraction of AHH1 cells. The doubling dose determined to be 838 ng/ml. A concentration of 30 micrograms/ml of this compound resembled the induced rate of 1 microM benzo (alpha) pryene.
将培养的AHH1人淋巴母细胞暴露于秘鲁用作药用植物和防腐剂的薄荷唇柱苣苔提取物中，以检测其诱变活性。对浓度为1、10、30和50微克/毫升的提取物进行为期三天的暴露，导致诱导突变率分别为每天每百万细胞1.02、1.79、2.21和3.80个突变，以6-硫鸟嘌呤抗性表示。10微克/毫升的浓度诱导的突变率是AHH1细胞自发突变率的11.9倍。确定加倍剂量为838纳克/毫升。该化合物30微克/毫升的浓度诱导率与1微摩尔苯并（α）芘相似。
