磷脂酰乙醇的合成——成年男性酗酒风险的潜在标志物。
Synthesis of phosphatidylethanol--a potential marker for adult males at risk for alcoholism.
作者信息
Mueller G C, Fleming M F, LeMahieu M A, Lybrand G S, Barry K J
机构信息
McArdle Laboratory for Cancer Research, University of Wisconsin-Madison 53706.
出版信息
Proc Natl Acad Sci U S A. 1988 Dec;85(24):9778-82. doi: 10.1073/pnas.85.24.9778.
Abstract
Phosphatidylethanol, whose synthesis is catalyzed by a phospholipase D in a transphosphatidylation reaction, is a unique metabolite of ethanol. Phorbol 12-tetradecanoate 13-acetate, a tumor-promoting phorbol ester and stimulator of protein kinase C, activates this enzyme in peripheral blood lymphocytes. This system has been developed into an assay for measuring the potential of this pathway in human subjects. A pilot study of phosphatidylethanol synthesis in lymphocytes of adult males who have both an alcohol dependency and a family history of alcoholism has revealed that the average potential for phosphatidylethanol synthesis in this population is significantly elevated over that of control subjects.
摘要
磷脂酰乙醇是乙醇的一种独特代谢产物，其合成由磷脂酶D在转磷脂酰基反应中催化。佛波醇12-十四烷酸酯13-乙酸酯是一种促肿瘤佛波酯和蛋白激酶C的刺激剂，可激活外周血淋巴细胞中的这种酶。该系统已发展成为一种用于测量人类受试者中该途径潜力的检测方法。一项对既有酒精依赖又有酗酒家族史的成年男性淋巴细胞中磷脂酰乙醇合成的初步研究表明，该人群中磷脂酰乙醇合成的平均潜力明显高于对照组。
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