Ross C A, Wright G E, Resh M D, Pearson R C, Snyder S H
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Proc Natl Acad Sci U S A. 1988 Dec;85(24):9831-5. doi: 10.1073/pnas.85.24.9831.
Brain src protooncogene is expressed in two forms, one identical to message in other tissues, and one containing an 18-nucleotide insert specific to brain. We have mapped mRNA for the two forms of src in rat brain with selective antisense oligonucleotide probes to the brain (src+) and peripheral (src-) forms. Fetal rat src mRNA levels were much higher in the central nervous system than any peripheral organ. In adult brain, src+ mRNA level was highest in the internal granular layer of the olfactory bulb, pyramidal cells of the hippocampus, granule cells of the dentate gyrus, and cerebellar granule cells. src+ and src- levels were similar in hindbrain, but src+ levels were higher than those of src- in forebrain. These distributions suggest that src+ may play roles in a number of neural processes, possibly including neuronal plasticity.
脑源原癌基因以两种形式表达，一种与其他组织中的信使核糖核酸相同，另一种含有特定于脑的18个核苷酸的插入片段。我们用针对脑（src+）和外周（src-）形式的选择性反义寡核苷酸探针，在大鼠脑中绘制了两种src形式的信使核糖核酸图谱。胎鼠src信使核糖核酸水平在中枢神经系统中比任何外周器官都高得多。在成体脑中，src+信使核糖核酸水平在嗅球的内颗粒层、海马体的锥体细胞、齿状回的颗粒细胞和小脑颗粒细胞中最高。src+和src-水平在后脑中相似，但src+水平在前脑中高于src-。这些分布表明src+可能在许多神经过程中发挥作用，可能包括神经元可塑性。
