Kansas State University, Manhattan, KS, USA.
euglena Co Ltd, Minato-ku, Tokyo, Japan.
Integr Cancer Ther. 2020 Jan-Dec;19:1534735419900555. doi: 10.1177/1534735419900555.
A colon cancer growth inhibitor partially purified from the isolated cell wall membrane fraction of , here referred to as membrane factor (CMF), was evaluated for its antitumor and immunomodulatory effects in cell culture and in a colon carcinoma mouse model. The CMF treatment dose- and time-dependently inhibited colon carcinoma cell growth in 2-dimensional cultures. Treatment with CMF also significantly inhibited the growth of colon carcinoma spheroids in 3-dimensional cell culture in coculture with T lymphocytes. In a mouse CT26 colon carcinoma peritoneal dissemination model, intraperitoneal injection of CMF (10 or 30 mg dry weight/kg body weight, every other day) dose-dependently and significantly attenuated the growth of tumor nodules via induction of tumor cell apoptosis. Evaluation of immune cell populations in ascites showed that CMF treatment tended to increase T lymphocytes but lower granulocyte populations. The present study suggests that the cell wall membrane fraction of contains a bioactive material that inhibits colon carcinoma growth via direct cell growth inhibition and stimulation of host antitumor immunity. Hence, it is suggested that the cell wall membrane extract or a bioactive substance in the extract is an attractive complementary medicine for cancer therapy.
一种从 中分离的细胞外壁膜部分纯化的结肠癌生长抑制剂，在这里称为细胞膜因子（CMF），在细胞培养和结肠癌小鼠模型中评价其抗肿瘤和免疫调节作用。CMF 处理剂量和时间依赖性地抑制二维培养中的结肠癌细胞生长。CMF 处理还显著抑制与 T 淋巴细胞共培养的三维细胞培养中的结肠癌球体生长。在 CT26 结肠癌腹膜扩散模型中，腹腔内注射 CMF（10 或 30mg 干重/千克体重，每隔一天）剂量依赖性地通过诱导肿瘤细胞凋亡显著抑制肿瘤结节的生长。对腹水免疫细胞群体的评估表明，CMF 处理倾向于增加 T 淋巴细胞但降低粒细胞群体。本研究表明 细胞外壁膜部分含有一种生物活性物质，通过直接抑制细胞生长和刺激宿主抗肿瘤免疫来抑制结肠癌生长。因此，建议 细胞外壁膜提取物或提取物中的生物活性物质是一种有吸引力的癌症治疗辅助药物。
