Taylor Dean M, Olds Cassandra L, Haney Reagan S, Torrevillas Brandi K, Luckhart Shirley
Department of Entomology, Plant Pathology and Nematology, University of Idaho, Moscow, ID, United States.
Department of Biological Sciences, University of Idaho, Moscow, ID, United States.
Front Microbiol. 2020 Jan 17;10:3024. doi: 10.3389/fmicb.2019.03024. eCollection 2019.
The larval environment of holometabolous insects determines many adult life history traits including, but not limited to, rate and success of development and adult lifespan and fecundity. The ancient stress signaling hormone abscisic acid (ABA), released by plants inundated with water and by leaf and root fragments in water, is likely ubiquitous in the mosquito larval environment and is well known for its wide ranging effects on invertebrate biology. Accordingly, ABA is a relevant stimulus and signal for mosquito development. In our studies, the addition of ABA at biologically relevant levels to larval rearing containers accelerated the time to pupation and increased death of pupae. We could not attribute these effects, however, to ABA-dependent changes in JH biosynthesis-associated gene expression, 20E titers or transcript patterns of genes. Adult females derived from ABA-treated larvae had reduced total protein content and significantly reduced post blood meal transcript expression of , effects that were consistent with variably reduced egg clutch sizes and oviposition success from the first through the third gonotrophic cycles. Adult female derived from ABA-treated larvae also exhibited reduced lifespans relative to controls. Collectively, these effects of ABA on life history traits are robust, durable and predictive of multiple impacts of an important malaria vector spreading to new malaria endemic regions.
全变态昆虫的幼虫环境决定了许多成虫的生活史特征，包括但不限于发育速度和成功率、成虫寿命以及繁殖力。古老的应激信号激素脱落酸（ABA）由被水淹没的植物以及水中的叶和根碎片释放，在蚊虫幼虫环境中可能普遍存在，并且以其对无脊椎动物生物学的广泛影响而闻名。因此，ABA是蚊虫发育的一种相关刺激和信号。在我们的研究中，向幼虫饲养容器中添加与生物学相关水平的ABA会加速化蛹时间并增加蛹的死亡。然而，我们无法将这些影响归因于与保幼激素生物合成相关的基因表达、20E滴度或基因转录模式中依赖ABA的变化。来自经ABA处理的幼虫的成年雌性总蛋白含量降低，并且血餐后转录本表达显著降低，这些影响与从第一个到第三个生殖营养周期中可变减少的卵块大小和产卵成功率一致。与对照相比，来自经ABA处理的幼虫的成年雌性寿命也缩短。总的来说，ABA对生活史特征的这些影响是强大、持久的，并且预示着一种重要的疟疾传播媒介扩散到新的疟疾流行地区的多种影响。
