Kagawa Yuki, Ozaki-Masuzawa Yori, Hosono Takashi, Seki Taiichiro
Department of Applied Life Science, Nihon University Graduate School of Bioresource Sciences, Fujisawa, Kanagawa 252-0880, Japan.
Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
Exp Ther Med. 2020 Feb;19(2):1536-1540. doi: 10.3892/etm.2019.8386. Epub 2019 Dec 27.
Garlic ( L.) has long been used as a medicinal food. Indeed, garlic and its constituents have been shown to possess potent regulatory activities in bodily functions, including blood coagulation, lipid metabolism, immunity and xenobiotic metabolism. In this study, we aimed to examine the anti-obesity effects of garlic oil and to elucidate the possible underlying mechanisms. For this purpose, garlic oil (GO; 80 mg/kg body weight, p.o.) or corn oil alone as a vehicle-control were administered to male Sprague-Dawley rats every other day for 10 weeks. The results revealed that GO administration significantly reduced body weight gain and white adipose tissue (WAT) mass, which had been increased by feeding on the AIN-76-based high-fat diet (60% kcal fat). Expired gas analysis was performed at 9 weeks following the GO administration to calculate fuel oxidation. GO administration enhanced O consumption during the dark period (at night) and increased energy expenditure through fat oxidation during the light period (daytime); however, carbohydrate oxidation remained unaltered. Western blot analysis revealed that GO administration increased UCP1 protein expression in brown adipose tissue (BAT). On the whole, the findings of this study indicated that GO suppressed body weight gain and WAT mass in the rat model of high-fat diet-induced obesity by increasing UCP1 expression and by enhancing fat oxidation and energy expenditure.
大蒜（L.）长期以来一直被用作药用食品。事实上，大蒜及其成分已被证明在身体功能方面具有强大的调节活性，包括血液凝固、脂质代谢、免疫和外源性物质代谢。在本研究中，我们旨在研究大蒜油的抗肥胖作用，并阐明其可能的潜在机制。为此，每隔一天给雄性斯普拉格 - 道利大鼠口服大蒜油（GO；80毫克/千克体重）或单独使用玉米油作为载体对照，持续10周。结果显示，给予GO可显著降低体重增加和白色脂肪组织（WAT）质量，这些指标在基于AIN - 76的高脂饮食（60%千卡脂肪）喂养后有所增加。在给予GO 9周后进行呼出气体分析以计算燃料氧化。给予GO可增强黑暗期（夜间）的氧气消耗，并通过白天的脂肪氧化增加能量消耗；然而，碳水化合物氧化保持不变。蛋白质印迹分析表明，给予GO可增加棕色脂肪组织（BAT）中UCP1蛋白的表达。总体而言，本研究结果表明，GO通过增加UCP1表达、增强脂肪氧化和能量消耗，抑制了高脂饮食诱导肥胖大鼠模型中的体重增加和WAT质量。
