State Key Laboratory of Medicinal Chemical biology, College of Pharmacy, Nankai University, Tianjin, 300071, China.
College of Chemistry, Nankai University, Tianjin, 300071, China.
Angew Chem Int Ed Engl. 2020 Apr 6;59(15):6263-6267. doi: 10.1002/anie.202000299. Epub 2020 Mar 2.
Fabrication of zeolite-like metal-organic frameworks (ZMOFs) for advanced applications, such as enzyme immobilization, is of great interest but is a great synthetic challenge. Herein, we have developed a new strategy using proteins as structure-directed agents to direct the formation of new ZMOFs that can act as versatile platforms for the in situ encapsulation of proteins under ambient conditions. Notably, protein incorporation directs the formation of a ZMOF with a sodalite (sod) topology instead of a non-porous diamondoid (dia) topology under analogous synthetic conditions. Histidines in proteins play a crucial role in the observed templating effect. Modulating histidine content thereby influenced the resultant MOF product (from dia to dia + sod mixture and, ultimately, to sod MOF). Moreover, the resulting ZMOF-incorporated proteins preserved their activity even after exposure to high temperatures and organic solvents, demonstrating their potential for biocatalysis and biopharmaceutical applications.
沸石样金属有机骨架（ZMOFs）的制备对于酶固定等先进应用具有重要意义，但合成具有挑战性。在此，我们开发了一种新策略，使用蛋白质作为结构导向剂来指导新型 ZMOFs 的形成，这些 ZMOFs 可以作为在环境条件下原位封装蛋白质的通用平台。值得注意的是，与类似的合成条件下形成非多孔类金刚石（dia）拓扑结构的 ZMOFs 相比，蛋白质的掺入指导了 sodalite（sod）拓扑结构的 ZMOF 的形成。蛋白质中的组氨酸在观察到的模板效应中起着关键作用。调节组氨酸含量从而影响了所得 MOF 产物（从 dia 到 dia + sod 混合物，最终到 sod MOF）。此外，即使在高温和有机溶剂暴露后，所得到的包含 ZMOF 的蛋白质仍保留其活性，这表明它们在生物催化和生物制药应用中的潜力。
