IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain.
Universitat Autònoma de Barcelona, Cerdanyola del Vallès, 08193, Bellaterra, Barcelona, Spain.
Vet Res. 2020 Feb 3;51(1):7. doi: 10.1186/s13567-020-0738-8.
Glaesserella (formerly Haemophilus) parasuis causes Glässer's disease, which results in high economic loss in the swine industry. To understand the polymicrobial interactions of G. parasuis and the nasal microbiota, the statistical association patterns of nasal colonizing bacteria with virulent and non-virulent strains of G. parasuis were studied accounting for the farm management practices as potential risk factors for the occurrence of Glässer's disease. The nasal microbiota from 51 weaned-piglets from four farms with Glässer's disease and three farms with no respiratory diseases was previously characterized and included in this study. The presence of virulent and/or non-virulent G. parasuis strains in the nasal cavities was determined in order to establish the potential association with other members of the nasal microbiota. Multivariate logistic and linear regression models were performed among the various members of nasal microbiota and G. parasuis. The multi-site production system and disease presence in the farm were both significantly associated with the presence of G. parasuis virulent strains in the nose of the piglets. Differential bacterial associations were observed with virulent or non-virulent G. parasuis. Chitinophagaceae, Corynebacteriaceae and Corynebacterium were positively associated with the virulent G. parasuis strains, while Enterobacteriaceae, Peptostreptococcaceae, Clostridium XI, and Escherichia/Shigella were negatively associated with virulent G. parasuis. On the other hand, Flavobacteriaceae, Planobacterium, and Phascolarctobacterium were positively associated with the non-virulent G. parasuis strains, while Rikenellaceae, Enterococcaceae, Odoribacter, and Corynebacterium were negatively associated with non-virulent G. parasuis. In conclusion, the nasal microbiota communities showed variations in the association with the G. parasuis strains type.
革拉瑟氏菌（以前称为嗜血杆菌）引起格拉泽氏病，给养猪业造成了巨大的经济损失。为了了解革拉瑟氏菌与鼻腔微生物群的多微生物相互作用，本研究针对农场管理实践这一潜在的格拉泽氏病发病风险因素，研究了鼻腔定植细菌与毒力和非毒力革拉瑟氏菌株之间的统计关联模式。本研究中包含了先前从四个患有格拉泽氏病和三个无呼吸道疾病的农场的 51 头断奶仔猪的鼻腔微生物群进行的特征描述。为了确定与鼻腔微生物群其他成员的潜在关联，确定了鼻腔中是否存在毒力和/或非毒力革拉瑟氏菌菌株。对鼻腔微生物群的各种成员与革拉瑟氏菌之间进行了多变量逻辑和线性回归模型分析。多地点生产系统和农场疾病的存在与仔猪鼻腔中革拉瑟氏菌毒力菌株的存在显著相关。观察到革拉瑟氏菌毒力或非毒力菌株与细菌的差异关联。噬几丁质菌科、棒状杆菌科和棒状杆菌属与毒力革拉瑟氏菌呈正相关，而肠杆菌科、消化链球菌科、梭菌 XI 属和大肠埃希氏菌/志贺氏菌属与毒力革拉瑟氏菌呈负相关。另一方面，黄杆菌科、Planobacterium 和 Phascolarctobacterium 与非毒力革拉瑟氏菌呈正相关，而 Rikenellaceae、肠球菌科、Odoribacter 和棒状杆菌属与非毒力革拉瑟氏菌呈负相关。总之，鼻腔微生物群群落与革拉瑟氏菌菌株类型的关联存在差异。
