School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 210089, PR China.
School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 210089, PR China.
Colloids Surf B Biointerfaces. 2020 May;189:110810. doi: 10.1016/j.colsurfb.2020.110810. Epub 2020 Jan 20.
In this study, a versatile doxorubicin (DOX)-loaded yolk-shell nano-particles (HMCMD) assembled with manganese dioxide (MnO) as the core and copper sulfide (HMCuS) as the mesoporous (∼ 6.4 nm) shell, was designed and synthesized. The resulting HMCMD possess excellent photothermal conversion efficiency. The DOX release from the yolk-shell nanoparticles could be promoted by laser irradiation, which increased the chemotherapy of DOX. Meanwhile, Mn could be released from the HMCMD through a redox reaction between MnO and abundant glutathione (GSH) in tumor cells. The released Mn could promote the decomposition of the intracellular hydrogen peroxide (HO) by Fenton-like reaction to generate the highly toxic hydroxyl radicals (·OH), thus exhibiting the effective chemodynamic therapy (CDT). Additionally, the efficiency of Mn-mediated CDT could be effectively enhanced by NIR irradiation. Further modification of polyethylene glycol (PEG) would improve the water solubility of the HMCMD to promote the uptake by MCF-7 cells. Hence, the HMCMD with synergistic effects of chemotherapy and chemodynamic/photothermal therapy would provide an alternative strategy in antitumor research.
在这项研究中，设计并合成了一种多功能阿霉素（DOX）负载的蛋黄壳纳米粒子（HMCMD），由二氧化锰（MnO）作为核和硫化铜（HMCuS）作为介孔（∼6.4nm）壳。所得的 HMCMD 具有优异的光热转换效率。激光照射可以促进蛋黄壳纳米粒子中 DOX 的释放，从而增加 DOX 的化疗效果。同时，Mn 可以通过 MnO 与肿瘤细胞中丰富的谷胱甘肽（GSH）之间的氧化还原反应从 HMCMD 中释放出来。释放的 Mn 可以通过芬顿样反应促进细胞内过氧化氢（HO）的分解，生成高毒性的羟基自由基（·OH），从而表现出有效的化学动力学治疗（CDT）。此外，近红外（NIR）照射可以有效增强 Mn 介导的 CDT 效率。进一步修饰聚乙二醇（PEG）可以提高 HMCMD 的水溶性，促进 MCF-7 细胞的摄取。因此，具有化学动力学/光热治疗协同作用的 HMCMD 将为抗肿瘤研究提供一种替代策略。
