College of Dental Medicine, Western University of Health Sciences, Pomona, CA, U.S.A.
Anticancer Res. 2020 Feb;40(2):751-758. doi: 10.21873/anticanres.14006.
BACKGROUND/AIM: This study investigated a novel combined therapy of rosmarinic acid (RA)/blue light on head and neck squamous cell carcinoma (HNSCC) cell proliferation in vitro.
HNSCC cells were exposed to BL (500 mW/cm) for 90 s, and incubated with 80 μg/ml RA for 1 hour. Cell viability was determined after 24 h using WST-1 assay. Western blot was used to detect treatment-induced changes in epidermal growth factor receptor (EGFR) activation. Hydrogen peroxide (HO) and nitric oxide levels were quantified using CM-HDCFH-DA assays. Apoptosis was assessed using Annexin V/PI staining and flow cytometry.
RA/blue light treatment resulted in a significant reduction in cell viability, EGFR activation and HO levels in all HNSCC cell lines. However, no significant changes in NO production or apoptosis induction were found.
RA/blue light effectively decreased HNSCC cell proliferation through reduction in EGFR activation and HO production, and not via induction of apoptosis.
背景/目的：本研究旨在探讨迷迭香酸（RA）/蓝光联合治疗对头颈部鳞状细胞癌（HNSCC）细胞体外增殖的影响。
将 HNSCC 细胞暴露于 BL（500 mW/cm）90 s，并在 80 μg/ml RA 中孵育 1 小时。使用 WST-1 测定法在 24 h 后测定细胞活力。使用 Western blot 检测治疗诱导的表皮生长因子受体（EGFR）激活的变化。使用 CM-HDCFH-DA 测定法定量测定过氧化氢（HO）和一氧化氮（NO）水平。通过 Annexin V/PI 染色和流式细胞术评估细胞凋亡。
RA/蓝光处理导致所有 HNSCC 细胞系的细胞活力、EGFR 激活和 HO 水平显著降低。然而，NO 产生或细胞凋亡诱导没有明显变化。
RA/蓝光通过降低 EGFR 激活和 HO 产生有效抑制 HNSCC 细胞增殖，而不是通过诱导细胞凋亡。
