Department of Orthopedics and Trauma, Medical University Graz, Graz, Austria.
Division of Biomedical Research, Medical University Graz, Graz, Austria.
Sci Rep. 2020 Feb 3;10(1):1682. doi: 10.1038/s41598-020-58742-9.
In this study, different surface modifications were performed on a Cobalt-Chrome-Molybdenum (CoCrMo) alloy and the effects on cell viability and cytotoxicity as well as the adhesion potential of human osteoblasts (hFOB) and their inflammation reaction were investigated in vitro. CoCrMo discs were coated with TiN, with polished and porous coated surfaces, or with pure titanum (cpTi) surfaces and examined by Scanning Electron Microscopy to evaluate surface modifications. In vitro cell viability, adhesion behaviour, and expression of inflammation markers of hFOB human osteoblasts were measured via CellTiter-Glo, CytoTox, ELISA, and RT-PCR respectively. All results were compared to CoCrMo without surface modifications. The biocompatibility data showed high compatibility for the TiN hard coatings. Likewise, the porous surface coating increased cell viability significantly, compared to an untreated CoCrMo alloy. None of the investigated materials influenced cytotoxicity. Different surface modifications did not influence expression of fibronectin, although TiN, porous surface coatings and polished surfaces showed highly significant reductions in integrin subunit expression. In addition to the regulation of adhesion potential these three surfaces stimulated an anti-inflammatory response by osteocytes. Improved biocompatibility and adhesion properties may contribute to better osteointegration of prosthetics.
本研究对钴铬钼（CoCrMo）合金进行了不同的表面改性，并在体外研究了其对细胞活力和细胞毒性的影响，以及人成骨细胞（hFOB）的黏附潜力及其炎症反应。CoCrMo 圆盘经过 TiN、抛光和多孔涂层表面处理，或纯钛（cpTi）表面处理，并通过扫描电子显微镜进行评估。通过 CellTiter-Glo、CytoTox、ELISA 和 RT-PCR 分别测量 hFOB 人成骨细胞的体外细胞活力、黏附行为和炎症标志物的表达。所有结果均与未经表面改性的 CoCrMo 进行比较。生物相容性数据表明 TiN 硬涂层具有很高的相容性。同样，与未处理的 CoCrMo 合金相比，多孔表面涂层显著提高了细胞活力。研究中没有一种材料影响细胞毒性。不同的表面改性并不影响纤连蛋白的表达，尽管 TiN、多孔表面涂层和抛光表面的整合素亚基表达显著降低。除了调节黏附潜力外，这三种表面还通过成骨细胞刺激抗炎反应。更好的生物相容性和黏附性能可能有助于改善假体的骨整合。
