Paulitsch-Fuchs Astrid H, Wolrab Lukas, Eck Nicole, Dyer Nigel P, Bödendorfer Benjamin, Lohberger Birgit
Biomedical Sciences, University of Applied Sciences Carinthia, 9020 Klagenfurt, Austria.
Diagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, 8010 Graz, Austria.
J Funct Biomater. 2021 May 18;12(2):36. doi: 10.3390/jfb12020036.
One of the most serious complications following joint replacement surgeries are periprosthetic infections (PIs) arising from the adhesion of bacteria to the artificial joint. Various types of titanium-aluminum-vanadium (TiAl6V4) alloy surface modifications (coatings with silver (Ag), titanium nitride (TiN), pure titanium (cpTi), combinations of cpTi and hydroxyapatite (HA), combinations of cpTi and tricalcium phosphate (TCP), and a rough-blasted surface of TiAl6V4) have been investigated to assess their effects on biofilm development. Biofilms were grown, collected, and analyzed after 48 h to measure their protein and glucose content and the cell viability. Biofilm-associated genes were also monitored after 48 h of development. There was a distinct difference in the development of staphylococcal biofilms on the surfaces of the different types of alloy. According to the findings of this study, the base alloy TiAl6V4 and the TiN-coated surface are the most promising materials for biofilm reduction. Rough surfaces are most favorable when it comes to bacterial infections because they allow an easy attachment of pathogenic organisms. Of all rough surfaces tested, rough-blasted TiAl6V4 was the most favorable as an implantation material; all the other rough surfaces showed more distinct signs of inducing the development of biofilms which displayed higher protein and polysaccharide contents. These results are supported by RT-qPCR measurements of biofilm associated genes for (, , , , , ) and (, ).
关节置换手术后最严重的并发症之一是细菌粘附到人工关节上引发的假体周围感染（PIs）。人们研究了各种类型的钛铝钒（TiAl6V4）合金表面改性方法（涂银（Ag）、氮化钛（TiN）、纯钛（cpTi）、cpTi与羟基磷灰石（HA）的组合、cpTi与磷酸三钙（TCP）的组合以及TiAl6V4的粗化喷砂表面），以评估它们对生物膜形成的影响。生物膜生长48小时后进行收集和分析，以测量其蛋白质和葡萄糖含量以及细胞活力。生物膜形成48小时后还监测了与生物膜相关的基因。不同类型合金表面葡萄球菌生物膜的形成存在明显差异。根据本研究的结果，基础合金TiAl6V4和TiN涂层表面是减少生物膜形成最有前景的材料。粗糙表面在细菌感染方面最有利，因为它们便于致病生物附着。在所有测试的粗糙表面中，粗化喷砂的TiAl6V4作为植入材料最有利；所有其他粗糙表面显示出更明显的诱导生物膜形成的迹象，这些生物膜显示出更高的蛋白质和多糖含量。这些结果得到了对生物膜相关基因（、、、、、）和（、）进行的RT-qPCR测量的支持。
