Lohberger Birgit, Eck Nicole, Glaenzer Dietmar, Kaltenegger Heike, Leithner Andreas
Department of Orthopedics and Trauma, Medical University Graz, 8036 Graz, Austria.
Materials (Basel). 2021 Mar 23;14(6):1574. doi: 10.3390/ma14061574.
Osteogenic cells are strongly influenced in their behaviour by the surface properties of orthopaedic implant materials. Mesenchymal stem and progenitor cells (MSPCs) migrate to the bone-implant interface, adhere to the material surface, proliferate and subsequently differentiate into osteoblasts, which are responsible for the formation of the bone matrix. Five surface topographies on titanium aluminium vanadium (TiAlV) were engineered to investigate biocompatibility and adhesion potential of human osteoblasts and the changes in osteogenic differentiation of MSPCs. Elemental analysis of TiAlV discs coated with titanium nitride (TiN), silver (Ag), roughened surface, and pure titanium (cpTi) surface was analysed using energy-dispersive X-ray spectroscopy and scanning electron microscopy. In vitro cell viability, cytotoxicity, adhesion behaviour, and osteogenic differentiation potential were measured via CellTiter-Glo, CytoTox, ELISA, Luminex technology, and RT-PCR respectively. The Ag coating reduced the growth of osteoblasts, whereas the viability of MSPCs increased significantly. The roughened and the cpTi surface improved the viability of all cell types. The additive coatings of the TiAlV alloy improved the adhesion of osteoblasts and MSPCs. With regard to the osteogenic differentiation potential, an enhanced effect has been demonstrated, especially in the case of roughened and cpTi coatings.
成骨细胞的行为受到骨科植入材料表面特性的强烈影响。间充质干祖细胞（MSPCs）迁移至骨-植入物界面，粘附于材料表面，增殖并随后分化为成骨细胞，而成骨细胞负责骨基质的形成。对钛铝钒（TiAlV）的五种表面形貌进行了设计，以研究人成骨细胞的生物相容性和粘附潜力以及MSPCs成骨分化的变化。使用能量色散X射线光谱和扫描电子显微镜对涂覆有氮化钛（TiN）、银（Ag）、粗糙表面和纯钛（cpTi）表面的TiAlV圆盘进行了元素分析。分别通过CellTiter-Glo、CytoTox、ELISA、Luminex技术和RT-PCR测量体外细胞活力、细胞毒性、粘附行为和成骨分化潜力。Ag涂层抑制了成骨细胞的生长，而MSPCs的活力显著增加。粗糙表面和cpTi表面提高了所有细胞类型的活力。TiAlV合金的添加剂涂层改善了成骨细胞和MSPCs的粘附。关于成骨分化潜力，已证明有增强作用，尤其是在粗糙表面和cpTi涂层的情况下。
