Institute of Animal Science, Chinese Academy of Agricultural Sciences, (CAAS), Beijing, China.
Sci Rep. 2020 Feb 4;10(1):1750. doi: 10.1038/s41598-020-58349-0.
The growth and development of embryonic skeletal muscle plays a crucial role in sheep muscle mass. But proteomic analyses for embryonic skeletal development in sheep had been little involved in the past research. In this study, we explored differential abundance proteins during embryonic skeletal muscle development by the tandem mass tags (TMT) and performed a protein profile analyses in the longissimus dorsi of Chinese merino sheep at embryonic ages Day85 (D85N), Day105 (D105N) and Day135 (D135N). 5,520 proteins in sheep embryonic skeletal muscle were identified, and 1,316 of them were differential abundance (fold change ≥1.5 and p-value < 0.05). After the KEGG enrichment analyses, these differential abundance proteins were significant enriched in the protein binding, muscle contraction and energy metabolism pathways. After validation of the protein quantification with the parallel reaction monitoring (PRM), 41% (16/39) significant abundance proteins were validated, which was similar to the results of protein quantification with TMT. All results indicated that D85N to D105N was the stage of embryonic muscle fibers proliferation, while D105N to D135N was the stage of their hypertrophy. These findings provided a deeper understanding of the function and rules of proteins in different phases of sheep embryonic skeletal muscle growth and development.
胚胎骨骼肌的生长和发育对绵羊肌肉质量起着至关重要的作用。但过去的研究中，很少涉及绵羊胚胎骨骼发育的蛋白质组学分析。在这项研究中，我们通过串联质量标签（TMT）探索了胚胎骨骼肌发育过程中的差异丰度蛋白，并对中国美利奴绵羊胚胎 85 日龄（D85N）、105 日龄（D105N）和 135 日龄（D135N）背最长肌的蛋白质谱进行了分析。在绵羊胚胎骨骼肌中鉴定出 5520 种蛋白质，其中 1316 种为差异丰度蛋白（倍数变化≥1.5，p 值<0.05）。经过 KEGG 富集分析，这些差异丰度蛋白在蛋白质结合、肌肉收缩和能量代谢途径中显著富集。经平行反应监测（PRM）验证蛋白定量后，有 41%（16/39）的显著丰度蛋白得到验证，与 TMT 定量结果相似。所有结果表明，D85N 到 D105N 是胚胎肌纤维增殖的阶段，而 D105N 到 D135N 是肌纤维肥大的阶段。这些发现为深入了解绵羊胚胎骨骼肌生长和发育不同阶段蛋白质的功能和规律提供了依据。
