Hu Yang, Wang Liping, Li Zhixing, Wan Zirui, Shao Mingjie, Wu Shaobin, Wang Guo
Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, People's Republic of China.
Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, People's Republic of China.
Onco Targets Ther. 2019 Dec 31;12:11609-11621. doi: 10.2147/OTT.S231941. eCollection 2019.
Colorectal cancer (CRC) is a common human malignancy. The aims of this study are to investigate the gene expression profile of CRC and to explore potential strategy for CRC diagnosis, therapy and prognosis.
We use affy and Limma package of Bioconductor R to do differential expression genes (DEGs) and differential expression lncRNAs (DELs) analysis from the gene datasets (GSE8671, GSE21510, GSE32323, GSE39582 and TCGA) respectively. Then, DEGs were analyzed by GO and KEGG pathway and Kaplan-Meier survival curve and Cox regression analyses were used to find aberrantly expressed genes associated with survival outcome of CRC patients. Real-time PCR assay was used to verify the aberrantly expressed genes expression in CRC samples.
306 up-regulation and 213 down-regulation common DEGs were found. A total of 485 DELs were identified, of which 241 up-regulated and 244 down-regulated. Then, GO and KEGG pathway analyses showed that DEGs were involved in cell cycle, mineral absorption, DNA replication, and Nitrogen metabolism. Among them, Kaplan-Meier survival curve and Cox regression analyses revealed that CDC6, CDC45, ORC6 and SNHG7 levels were significantly associated with survival outcome of CRC patients. Finally, real-time PCR assay was used to verify that the CDC6, CDC45, ORC6 and SNHG7 expression were up-regulated in 198 CRC samples compared with the expression levels in individual-matched adjacent mucosa samples.
CDC6, CDC45, ORC6 and SNHG7 are implicated in CRC initiation and progression and could be explored as potential diagnosis, therapy and prognosis targets for CRC.
结直肠癌（CRC）是一种常见的人类恶性肿瘤。本研究的目的是调查CRC的基因表达谱，并探索CRC诊断、治疗和预后的潜在策略。
我们分别使用Bioconductor R的affy和Limma软件包对基因数据集（GSE8671、GSE21510、GSE32323、GSE39582和TCGA）进行差异表达基因（DEGs）和差异表达长链非编码RNA（DELs）分析。然后，对DEGs进行GO和KEGG通路分析，并使用Kaplan-Meier生存曲线和Cox回归分析来寻找与CRC患者生存结果相关的异常表达基因。采用实时PCR检测法验证CRC样本中异常表达基因的表达情况。
共发现306个上调和213个下调的常见DEGs。共鉴定出485个DELs，其中241个上调，244个下调。然后，GO和KEGG通路分析表明，DEGs参与细胞周期、矿物质吸收、DNA复制和氮代谢。其中，Kaplan-Meier生存曲线和Cox回归分析显示，CDC6、CDC45、ORC6和SNHG7水平与CRC患者的生存结果显著相关。最后，采用实时PCR检测法验证，与个体匹配的相邻黏膜样本相比，198个CRC样本中CDC6、CDC45、ORC6和SNHG7的表达上调。
CDC6、CDC45、ORC6和SNHG7与CRC的发生和发展有关，可作为CRC潜在的诊断、治疗和预后靶点进行探索。
