Tito Annalisa, Minale Massimiliano, Riccio Salvatore, Grieco Fabrizia, Colucci Maria Gabriella, Apone Fabio
Arterra Bioscience Srl, Naples, 80142, Italy.
Farmaceutici Damor S.p.A., Naples 80145, Italy.
Clin Cosmet Investig Dermatol. 2020 Jan 14;13:21-30. doi: 10.2147/CCID.S216391. eCollection 2020.
Chronic skin lesions represent a problem of increasing occurrence, mostly due to the global ageing of the world population. Research in skin care and dermatology is constantly looking for new non-invasive solutions, preferably those based on the use of natural certified products, able to accelerate the spontaneous skin repair mechanisms and without altering the skin normal appearance and functionality. The wound healing process in the skin is finely regulated by several factors and orchestrated mechanisms, which modulate the progression and the fitting of different consequent phases, including haemostasis, inflammation, cell proliferation and tissue remodelling. It was previously shown that a patented aqueous extract was able to trigger the skin repair process by stimulating new tissue growth and reducing the expression levels of inflammatory mediators, such as IL-6, TNFα, prostaglandin E2, and nitric oxide.
Scratch assay was performed in Human Dermal Fibroblasts (HDF). The production of fibronectin was measured by gene expression, protein quantification and localization using specific antibodies in HDF. The polymerization of actin was measured using rhodamin-phalloidin in HDF. The epidermal lipid content was estimated in HaCaT (human spontaneously immortalized keratinocytes) using Nile Red staining and the increasing GBA gene expression and activity was demonstrated by RT-PCR and enzymatic activity assay.
In the present study, it was demonstrated that the extract enhanced cell migration inducing the synthesis of fibronectin, new actin polymerization and stimulating the expression of the Hyaluronan Synthase 2. Furthermore it improved the restoration of the epidermal barrier stimulating lipid synthesis.
In conclusion, we demonstrated that the extract possessed promising potential to be developed as a wound healing promoting agent in skin care and dermatology.
慢性皮肤损伤的发生率日益增加，这主要归因于全球人口老龄化。皮肤护理和皮肤病学领域的研究一直在寻找新的非侵入性解决方案，最好是基于使用天然认证产品的方案，这些方案能够加速皮肤的自发修复机制，同时不改变皮肤的正常外观和功能。皮肤中的伤口愈合过程受到多种因素和精心协调的机制的精细调控，这些因素和机制调节着不同后续阶段的进展和适配，包括止血、炎症、细胞增殖和组织重塑。先前的研究表明，一种专利水性提取物能够通过刺激新组织生长和降低炎症介质（如白细胞介素-6、肿瘤坏死因子α、前列腺素E2和一氧化氮）的表达水平来触发皮肤修复过程。
在人皮肤成纤维细胞（HDF）中进行划痕试验。通过基因表达、蛋白质定量以及使用特异性抗体在HDF中进行定位来测量纤连蛋白的产生。使用罗丹明-鬼笔环肽在HDF中测量肌动蛋白的聚合。使用尼罗红染色在HaCaT（人自发永生化角质形成细胞）中估计表皮脂质含量，并通过逆转录-聚合酶链反应（RT-PCR）和酶活性测定来证明葡萄糖脑苷脂酶（GBA）基因表达和活性的增加。
在本研究中，已证明该提取物可增强细胞迁移，诱导纤连蛋白的合成、新的肌动蛋白聚合，并刺激透明质酸合酶2的表达。此外，它还通过刺激脂质合成改善了表皮屏障的恢复。
总之，我们证明该提取物具有作为皮肤护理和皮肤病学中促进伤口愈合剂开发的潜力。
