Alessio H M, Goldfarb A H, Cutler R G
Department of Physical Education, Health, and Sport Studies, Miami University, Oxford, Ohio 45056.
Am J Physiol. 1988 Dec;255(6 Pt 1):C874-7. doi: 10.1152/ajpcell.1988.255.6.C874.
Previous work has shown that high-intensity (HI) exercise results in total body increased production of lipid peroxidation. These results suggest that exercise induces a higher level of oxidative stress in muscles leading to the production of various peroxides and aldehydes that are potentially toxic to cells. However, these past studies were carried out only with subjects that were exercised to exhaustion or at a very high intensity. In this paper, we report how a moderate-intensity (MI) exercise, which is more normally experienced, as well as a HI acute bout of exercise influenced oxidative stress-related reactions by measuring malonaldehyde (MDA) and lipid hydroperoxides (LH) in red vastus, white vastus, and soleus muscle. The muscles from untrained male Sprague-Dawley rats were removed immediately after either a HI 1-min run at 45 m/min (n = 8) or a 20-min MI run at 20 m/min (n = 8) and compared with a control group that did not run. MI exercise resulted in a 90% increase in MDA in white vastus and a 62% increase in red vastus muscle (P less than 0.05). HI exercise resulted in a 157, 167, and 83% increase in MDA in white vastus, red vastus, and soleus muscle, respectively. LH values in red and white vastus after HI exercise increased an average of 33%, but this proved not to be statistically significant. These results confirm earlier studies that HI exercise does increase MDA in skeletal muscle, and when intensity of exercise is considerably lowered, elevated MDA is still found but at a relatively lesser amount.
先前的研究表明，高强度（HI）运动可导致全身脂质过氧化产物生成增加。这些结果表明，运动可诱导肌肉产生更高水平的氧化应激，从而导致各种过氧化物和醛类物质的生成，这些物质可能对细胞有毒性。然而，过去这些研究仅在运动至力竭或极高强度的受试者中进行。在本文中，我们报告了通过测量股直肌、股外侧肌和比目鱼肌中的丙二醛（MDA）和脂质氢过氧化物（LH），中等强度（MI）运动（这是更常见的运动强度）以及一次HI急性运动 bout 如何影响与氧化应激相关的反应。在未训练的雄性 Sprague-Dawley 大鼠以 45 米/分钟的速度进行 1 分钟 HI 跑步（n = 8）或以 20 米/分钟的速度进行 20 分钟 MI 跑步（n = 8）后，立即取出肌肉，并与未跑步的对照组进行比较。MI 运动导致股外侧肌中 MDA 增加 90%，股直肌中 MDA 增加 62%（P < 0.05）。HI 运动分别导致股外侧肌、股直肌和比目鱼肌中 MDA 增加 157%、167%和 83%。HI 运动后股直肌和股外侧肌中的 LH 值平均增加 33%，但这在统计学上无显著意义。这些结果证实了早期的研究，即 HI 运动确实会增加骨骼肌中的 MDA，并且当运动强度大幅降低时，仍会发现 MDA 升高，但升高幅度相对较小。
