Mansouri Vahid, Razzaghi Mohammadreza, Rostami-Nejad Mohammad, Rezaei-Tavirani Majid, Heidari Mohammad Hossein, Safari Saeed, Arjmand Babak, Rezaei-Tavirani Mostafa, Zali Alireza, Hamdieh Mostafa
Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2020 Summer;11(3):280-286. doi: 10.34172/jlms.2020.47. Epub 2020 Jun 21.
Photobiomodulation (PBM) is known as low-level laser (or light) therapy and is applied in different fields of medicine. However, it is required that its molecular and cellular mechanism be investigated. This study aims to assess the neuroprotective properties of PBM in the rat retina. GSE22818 was downloaded from Gene Expression Omnibus (GEO) and the regulation of the significant differentially expressed genes (DEGs) which are produced by light damage in the rat retina by the pretreatment of PBM application was assessed via network analysis and gene ontology enrichment. The 78 produced DEGs by light-damage in the rat retina were protected via PBM pretreatment action. Among these determined DEGs, 53 individuals were included in the main connected component of the constructed protein-protein interaction (PPI) network. Ccl2, Icam1, Cxcl10, Timp1, and Fos were determined as hub nodes. Eight clusters including 26 regulated biochemical pathways by PBM pretreatment were identified. The critical DEGs based on the action maps were introduced. The finding indicates that PBM treatment protects rat retina against light damage via the prevention of Fos, Ccl2, Icam1, Cxcl10, and Myc dysregulation.
光生物调节（PBM）被称为低强度激光（或光）疗法，并应用于医学的不同领域。然而，需要对其分子和细胞机制进行研究。本研究旨在评估PBM对大鼠视网膜的神经保护特性。从基因表达综合数据库（GEO）下载GSE22818，并通过网络分析和基因本体富集评估PBM预处理对大鼠视网膜光损伤产生的显著差异表达基因（DEG）的调控。PBM预处理可保护大鼠视网膜中由光损伤产生的78个DEG。在这些确定的DEG中，53个个体包含在构建的蛋白质-蛋白质相互作用（PPI）网络的主要连通组件中。Ccl2、Icam1、Cxcl10、Timp1和Fos被确定为枢纽节点。鉴定出包括26条经PBM预处理调节的生化途径的8个聚类。介绍了基于作用图谱的关键DEG。研究结果表明，PBM治疗通过预防Fos、Ccl2、Icam1、Cxcl10和Myc失调来保护大鼠视网膜免受光损伤。
