Arjmand Babak, Khodadoost Mahmood, Jahani Sherafat Somayeh, Rezaei Tavirani Mostafa, Ahmadi Nayebali, Okhovatian Farshad, Rezaei Tavirani Majid
Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2021 Oct 9;12:e59. doi: 10.34172/jlms.2021.59. eCollection 2021.
There are many documents about the significant role of low-level laser therapy (LLLT) in different processes such as regenerator medicine and bone formation. The aim of this study is to assess the role of LLLT in blood hemostasis in rats via bioinformatic investigation. The differentially expressed plasma proteins of treated rats via LLLT from the literature and the added 50 first neighbors were investigated via network analysis to find the critical dysregulated proteins and biological processes by using Cytoscape software, the STRING database, and ClueGO. A scale-free network including 55 nodes was constructed from queried and added first neighbor proteins. Fibrinogen gamma, fibrinogen alpha, and plasminogen were highlighted as the central genes of the analyzed network. Fibrinolysis was determined as the main group of biological processes that were affected by LLLT. Findings indicate that LLLT affects blood hemostasis which is an important point in approving the therapeutic application of LLLT and also in preventing its possible complication.
有许多文献论述了低强度激光疗法（LLLT）在再生医学和骨形成等不同过程中的重要作用。本研究旨在通过生物信息学研究评估LLLT在大鼠血液止血中的作用。通过网络分析，利用Cytoscape软件、STRING数据库和ClueGO，对文献中经LLLT处理的大鼠差异表达血浆蛋白以及新增的50个第一邻域蛋白进行研究，以找出关键的失调蛋白和生物学过程。从查询到的蛋白和新增的第一邻域蛋白构建了一个包含55个节点的无标度网络。纤维蛋白原γ、纤维蛋白原α和纤溶酶原被突出显示为分析网络的中心基因。纤维蛋白溶解被确定为受LLLT影响的主要生物学过程组。研究结果表明，LLLT会影响血液止血，这在批准LLLT的治疗应用以及预防其可能的并发症方面都是一个重要要点。
