来自[具体来源未提及]的一种tRNA结合蛋白的RNA适配体及其在进化过程中分布的同源对应物。

RNA Aptamers for a tRNA-Binding Protein from with Homologous Counterparts Distributed Throughout Evolution.

作者信息

Ohmori Senri, Wani Marina, Kitabatake Saki, Nakatsugawa Yuka, Ando Tadashi, Umehara Takuya, Tamura Koji

机构信息

Department of Biological Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.

Department of Applied Electronics, Tokyo University of Science, 6-3-1 Katsushika-ku, Tokyo 125-8585, Japan.

出版信息

Life (Basel). 2020 Feb 1;10(2):11. doi: 10.3390/life10020011.

DOI:10.3390/life10020011

PMID:32024042

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7175363/

Abstract

In the present selection study, we isolated and characterized RNA aptamers for a tRNA-binding protein (Trbp) from an extremophile archaeon . Trbp-like structures are frequently found not only in aminoacyl-tRNA synthetases but also in diverse types of proteins from different organisms. They likely arose early in evolution and have played important roles in evolution through interactions with key RNA structures. RNA aptamers specific for Trbp were successfully selected from a pool of RNAs composed of 60 nucleotides, including a random 30-nucleotide region. From the secondary structures, we obtained a shortened sequence composed of 21 nucleotides, of which the 3'-terminal single stranded CA nucleotides are essential for binding. This may be related to the initial evolutionary role of the universal CCA-3' terminus of tRNA in the interaction with Trbp-like structures.

摘要

在目前的筛选研究中，我们从一种嗜极古菌中分离并鉴定了一种与tRNA结合蛋白（Trbp）结合的RNA适配体。Trbp样结构不仅常见于氨酰tRNA合成酶中，也存在于来自不同生物体的多种蛋白质中。它们可能在进化早期就已出现，并通过与关键RNA结构的相互作用在进化过程中发挥了重要作用。从由60个核苷酸组成的RNA文库中成功筛选出了对Trbp具有特异性的RNA适配体，该文库包括一个30个核苷酸的随机区域。从二级结构中，我们获得了一个由21个核苷酸组成的缩短序列，其中3'-末端的单链CA核苷酸对于结合至关重要。这可能与tRNA普遍的CCA-3'末端在与Trbp样结构相互作用中的初始进化作用有关。