College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan, 250014, P.R. China.
Center of Research and Development, Bloomage Biotechnology Corporation Limited, Jinan, 250100, P.R. China.
Sci Rep. 2020 Feb 5;10(1):1858. doi: 10.1038/s41598-020-58992-7.
Hyaluronic acid (HA) is applied in a number of medical applications and HA of different molecular weight (Mw) are used in different pharmaceutical preparations. In determination of Mw by muti-angle laser light-scattering (MALS), refractive index increment (dn/dc) is an important parameter for accuracy. Herein, the influence of dn/dc on the Mw of HA in stroke-physiological saline solution is investigated by MALS in this work. Additionally, the Mw variation of HA in the manufacturing process of preparations is measured. It is shown that each HA sample corresponds to a specific value of dn/dc, which is varied from 1.38 to 1.74 L/g with the Mw increasing from 13.5 to 2840 kDa in solution. It is indicated by the results from both MALS approach and viscometry that appropriate dn/dc should be selected for Mw determination. In steam sterilization process of preparations at 121 °C, the Mw and conformation of HA can be accurately and rapidly determined by MALS. This work provides a precise method to determine the Mw of HA in the medical applications and preparation industries.
透明质酸（HA）在许多医学应用中得到应用，不同分子量（Mw）的 HA 用于不同的药物制剂。在多角度激光光散射（MALS）测定 Mw 时，折光指数增量（dn/dc）是准确性的一个重要参数。本工作通过 MALS 研究了 dn/dc 对中风生理盐水溶液中 HA 的 Mw 的影响。此外，还测量了制剂制备过程中 HA 的 Mw 变化。结果表明，每个 HA 样品对应于特定的 dn/dc 值，其在溶液中从 13.5 到 2840 kDa 时，从 1.38 到 1.74 L/g 变化。MALS 方法和粘度计的结果表明，应选择适当的 dn/dc 值来测定 Mw。在制剂于 121°C 进行蒸汽灭菌过程中，MALS 可快速准确地测定 HA 的 Mw 和构象。本工作为医疗应用和制剂行业中 HA 的 Mw 测定提供了一种精确的方法。
