Department of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, 422-8526, Japan.
Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, Chiba, 263-8522, Japan.
J Antibiot (Tokyo). 2020 May;73(5):320-323. doi: 10.1038/s41429-020-0281-x. Epub 2020 Feb 5.
Chaetomium globosum is a filamentous fungus from which we have previously isolated a series of interesting natural products. Here, we isolated a previously unknown natural product from the culture of C. globosum. Through spectroscopic and crystallographic characterization, we determined the compound to be a new dimerized azaphilone-type product which we termed cochliodone J (1). Furthermore, our investigation into the biological activity of the natural product determined that 1 was cytotoxic to human cervix carcinoma HeLa cells with an IC of 17.3 µM. Lastly, a plausible biosynthetic mechanism for 1 is suggested based on our previous study on the biosynthesis of a closely related compound, cochliodone A (2).
球毛壳菌是一种丝状真菌，我们之前从该真菌中分离得到了一系列有趣的天然产物。在这里，我们从球毛壳菌的培养物中分离得到了一种以前未知的天然产物。通过光谱和晶体学表征，我们确定该化合物为一种新的二聚化吖啶酮型产物，我们将其命名为 Cochliodone J（1）。此外，我们对天然产物的生物活性研究表明，1 对人宫颈癌 HeLa 细胞具有细胞毒性，IC 为 17.3µM。最后，根据我们之前对密切相关化合物 Cochliodone A（2）生物合成的研究，提出了 1 的可能生物合成机制。
