Department of Neurology and Clinical Center for Neuroscience, Daping Hospital, Third Military Medical University (Army Medical University), Chongqing, China.
Basic Medical College, Third Military Medical University (Army Medical University), Chongqing, China.
Neurotox Res. 2020 Apr;37(4):1029-1035. doi: 10.1007/s12640-020-00161-9. Epub 2020 Feb 5.
Hyperphosphorylated tau is an important pathological agent in Alzheimer's disease (AD). Tau effluxes from the brain to the blood could potentially stimulate the production of naturally occurring antibodies (NAbs). We aimed to investigate whether NAbs to tau (NAbs-tau) was generated in human blood and to figure out the alteration of plasma NAbs-tau level in AD patients. About 192 AD patients and 192 age-matched and non-demented controls (NC) were enrolled in the present study. Immunofluorescence staining and western blot assays were used to confirm the existence of NAbs-tau in human blood. The plasma level of NAbs-tau in NC and AD group was analyzed by ELISA. Immunofluorescence staining and western blot assays confirmed the existence of NAbs-tau in human blood. However, no significant difference in the plasma level of NAbs-tau was observed between NC and AD group. Furthermore, the plasma level of NAbs-tau had no significant correlation with MMSE scores. The present study confirmed that NAbs-tau exists in human blood but does not differ in level between the NC and AD group. Plasma NAbs-tau is not a reliable biomarker for AD.
过度磷酸化的 tau 是阿尔茨海默病 (AD) 的重要病理因子。tau 从大脑流出到血液中，可能会刺激天然存在的抗体 (NAbs) 的产生。我们旨在研究人类血液中是否产生了针对 tau 的 NAbs（NAbs-tau），并确定 AD 患者血浆 NAbs-tau 水平的变化。本研究纳入了 192 名 AD 患者和 192 名年龄匹配且无痴呆的对照组（NC）。免疫荧光染色和 Western blot 分析用于确认人类血液中 NAbs-tau 的存在。通过 ELISA 分析 NC 和 AD 组血浆 NAbs-tau 水平。免疫荧光染色和 Western blot 分析证实了人类血液中 NAbs-tau 的存在。然而，NC 和 AD 组之间的 NAbs-tau 血浆水平没有显著差异。此外，NAbs-tau 血浆水平与 MMSE 评分无显著相关性。本研究证实 NAbs-tau 存在于人类血液中，但在 NC 和 AD 组之间水平没有差异。血浆 NAbs-tau 不是 AD 的可靠生物标志物。
