Tao Ran, Reboud Julien, Torun Hamdi, McHale Glen, Dodd Linzi E, Wu Qiang, Tao Kai, Yang Xin, Luo Jing Ting, Todryk Stephen, Fu Yongqing
Shenzhen Key Laboratory of Advanced Thin Films and Applications, College of Physics and Energy, Shenzhen University, 518060, Shenzhen, China.
Division of Biomedical Engineering, James Watt School of Engineering, University of Glasgow, Glasgow, G12 8LT, UK.
Lab Chip. 2020 Mar 3;20(5):1002-1011. doi: 10.1039/c9lc01189g.
Integration of microfluidics and biosensing functionalities on a single device holds promise in continuous health monitoring and disease diagnosis for point-of-care applications. However, the required functions of fluid handling and biomolecular sensing usually arise from different actuation mechanisms. In this work, we demonstrate that a single acoustofluidic device, based on a flexible thin film platform, is able to generate hybrid wave modes, which can be used for fluidic actuation (Lamb waves) and biosensing (thickness shear waves). On this integrated platform, we show multiple and sequential functions of mixing, transport and disposal of liquid volumes using Lamb waves, whilst the thickness bulk shear waves allow us to sense the chemotherapeutic Imatinib, using an aptamer-based strategy, as would be required for therapy monitoring. Upon binding, the conformation of the aptamer results in a change in coupled mass, which has been detected. This platform architecture has the potential to generate a wide range of simple sample-to-answer biosensing acoustofluidic devices.
将微流体和生物传感功能集成在单个设备上，有望实现即时医疗应用中的连续健康监测和疾病诊断。然而，流体处理和生物分子传感所需的功能通常源于不同的驱动机制。在这项工作中，我们证明了基于柔性薄膜平台的单个声流体设备能够产生混合波模式，可用于流体驱动（兰姆波）和生物传感（厚度剪切波）。在这个集成平台上，我们展示了利用兰姆波对液体体积进行混合、传输和处理的多种连续功能，而厚度体剪切波则使我们能够采用基于适配体的策略来检测化疗药物伊马替尼，这是治疗监测所必需的。结合后，适配体的构象导致耦合质量发生变化，这已被检测到。这种平台架构有潜力生成一系列简单的样品到答案的生物传感声流体设备。
