School of Integrative Plant Sciences, Bradfield Hall, Cornell University, Ithaca, NY, 14853, USA.
Department of Microbiology, Wing Hall, Cornell University, Ithaca, NY, 14853, USA.
Int J Syst Evol Microbiol. 2020 Mar;70(3):2137-2146. doi: 10.1099/ijsem.0.004029.
RP11 was isolated from forest soil following enrichment with 4-hydroxybenzoic acid. Cells of RP11 are aerobic, non-sporulating, exhibit swimming motility, and are rods (0.8 µm by 1.4 µm) that often occur as diplobacillus or in short chains (3-4 cells). Optimal growth on minimal media containing 4-hydroxybenzoic acid (µ=0.216 hr) occurred at 30 °C, pH 6.5 or 7.0 and 0% salinity. Comparative chemotaxonomic, genomic and phylogenetic analyses revealed the isolate was distinct from its closest relative type strains identified as LMG 27731, LMG 16225 and CF1. Strain RP11 is genetically distinct from , its closest relative, in terms of 16S rRNA gene sequence similarity (98.7%), genomic average nucleotide identity (94%) and DNA-DNA hybridization (56.7 %±2.8). The composition of fatty acids and substrate utilization pattern differentiated strain RP11 from its closest relatives, including growth on phthalic acid. Strain RP11 encoded the greatest number of aromatic degradation genes of all eleven closely related type strains and uniquely encoded a phthalic acid dioxygenase and paralog of the 3-hydroxybenzoate 4-monooxygenase. The only ubiquinone detected in strain RP11 was Q-8, and the major cellular fatty acids were C, 3OH-C, C cyclo, C cyclo ω8c, and summed feature 8 (C ω7c/ω6c). On the basis of this polyphasic approach, it was determined that strain RP11 represents a novel species from the genus for which the name sp. nov. is proposed. The type strain is RP11 (=DSM 110123=LMG 31517).
RP11 是从富含 4-羟基苯甲酸的森林土壤中分离出来的。RP11 细胞为需氧、非孢子形成、具有游动性，呈杆状（0.8µm×1.4µm），常呈双杆菌或短链（3-4 个细胞）。在含有 4-羟基苯甲酸的最小培养基中（µ=0.216 小时），最佳生长温度为 30°C，pH 值为 6.5 或 7.0，盐度为 0%。比较化学分类学、基因组和系统发育分析表明，该分离株与最接近的亲缘关系的模式菌株 LMG 27731、LMG 16225 和 CF1 不同。与最接近的亲缘关系 相比，菌株 RP11 在 16S rRNA 基因序列相似性（98.7%）、基因组平均核苷酸同一性（94%）和 DNA-DNA 杂交（56.7%±2.8%）方面具有遗传上的差异。脂肪酸组成和底物利用模式将菌株 RP11 与其最接近的亲缘关系区分开来，包括在邻苯二甲酸上的生长。菌株 RP11 编码的芳香族降解基因数量最多，所有 11 个密切相关的模式菌株中都有，并且独特地编码了邻苯二甲酸双加氧酶和 3-羟基苯甲酸 4-单加氧酶的旁系同源物。在菌株 RP11 中检测到的唯一的泛醌是 Q-8，主要的细胞脂肪酸是 C、3OH-C、C 环、C 环 ω8c 和总和特征 8（C ω7c/ω6c）。基于这种多相方法，确定菌株 RP11 代表了一个新的种，属于 属，建议使用新种名 sp. nov.。模式菌株为 RP11（=DSM 110123=LMG 31517）。
