Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou, 450046, China.
College of Animal Science, Tarim University, Alar, Xinjiang, 843300, China.
Exp Parasitol. 2020 Mar;210:107850. doi: 10.1016/j.exppara.2020.107850. Epub 2020 Feb 3.
Experimental rats are important animal models, and a history of pathogenic infections in these animals will directly affect the animal trial results. Enterocytozoon bieneusi is a ubiquitous potential pathogen transmitted via the fecal-oral route. To determine the prevalence and genotypic distributions of E. bieneusi in experimental rats in China, 291 fresh fecal samples were collected from four medical experimental animal centers. Enterocytozoon bieneusi was screened via nested-PCR amplification of the internal transcribed spacer (ITS) region. Of the rats tested, 4.8% (14/291) were positive for E. bieneusi. Five E. bieneusi ITS genotypes (four known: EbpA, EbpC, CHY1, and N; one novel: SHR1) were detected among 14 sequenced samples. The dominant E. bieneusi genotype was EbpA (50.0%, 7/14). In the phylogenetic analysis, genotypes EbpA and EbpC belonged to the previously described group 1, genotypes N and SHR1 belonged to group 2, and genotype CHY1 belonged to the novel group 12. To our knowledge, this is the first report of E. bieneusi in experimental laboratory rats in China. Infections with this pathogen must be monitored in laboratory animals, and quality control officers in the medical experimental centers should attempt to trace the pathogen's source and stop its transmission.
实验大鼠是重要的动物模型，这些动物的病原感染史将直接影响动物试验结果。肠微孢子虫是一种通过粪-口途径传播的普遍潜在病原体。为了确定中国实验大鼠中肠微孢子虫的流行情况和基因型分布，从四个医学实验动物中心采集了 291 份新鲜粪便样本。通过内部转录间隔区（ITS）区域的巢式 PCR 扩增筛选肠微孢子虫。在检测的大鼠中，4.8%（14/291）对肠微孢子虫呈阳性。在 14 个测序样本中检测到了 5 种肠微孢子虫 ITS 基因型（4 种已知：EbpA、EbpC、CHY1 和 N；1 种新型：SHR1）。优势肠微孢子虫基因型为 EbpA（50.0%，7/14）。在系统发育分析中，基因型 EbpA 和 EbpC 属于先前描述的第 1 组，基因型 N 和 SHR1 属于第 2 组，基因型 CHY1 属于新型第 12 组。据我们所知，这是中国实验实验室大鼠中首次报道肠微孢子虫。必须对实验动物中的这种病原体进行监测，医学实验中心的质量控制人员应尝试追踪病原体的来源并阻止其传播。
