State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China.
Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei, 430075, PR China.
Anal Chim Acta. 2020 Mar 8;1101:111-119. doi: 10.1016/j.aca.2019.12.030. Epub 2019 Dec 13.
We designed a new type of MIP-SERS substrate for specific and label-free detection of patulin (PAT), by combining molecular imprinting polymer (MIP) selectivity and SERS technology sensitivity. Initially, the solid substrate of PDMS/AAO was prepared using poly dimethylsiloxane (PDMS) concreted anodized aluminum oxide (AAO) template. Then moderate Au was sputtered on the surface of PDMS/AAO to obtain Au/PDMS/AAO SERS substrate. Based on the HRP enzyme initiated in situ polymerization on the Au/PDMS/AAO, the MIP-SERS substrate was successfully synthesized with selective polymer and high tense of SERS "hot spots". The new MIP-SERS substrate showed strong SERS enhancement effect and good selectivity for PAT. Besides, the results showed that the method owned a linear range from 5 × 10 to 10 M with the limit of detection (LOD) of 8.5 × 10 M (S/N = 3) for PAT. The proposed method also exhibited acceptable reproducibility (relative standard deviation, RSD = 4.7%)，good stability (Raman intensity is above 80% after two weeks) and recoveries from 96.43% to 112.83% with the average RSD of 6.3%. The substrate is easy to use without complex sample pretreatment, which makes it a potential candidate as a rapid and sensitive detection method in food samples.
我们设计了一种新型的 MIP-SERS 基底，用于特异性和无标记检测棒曲霉素 (PAT)，方法是结合分子印迹聚合物 (MIP) 的选择性和 SERS 技术的灵敏度。首先，使用聚二甲基硅氧烷 (PDMS) 浇注阳极氧化铝 (AAO) 模板制备 PDMS/AAO 固体基底。然后在 PDMS/AAO 表面适度溅射 Au，以获得 Au/PDMS/AAO SERS 基底。基于 HRP 酶在 Au/PDMS/AAO 上引发的原位聚合，成功合成了具有选择性聚合物和高 SERS“热点”密度的 MIP-SERS 基底。新型 MIP-SERS 基底对 PAT 表现出强烈的 SERS 增强效应和良好的选择性。此外，结果表明，该方法在 5×10 至 10 M 范围内具有线性关系，检测限 (LOD) 为 8.5×10 M (S/N=3)。该方法还表现出可接受的重现性 (相对标准偏差，RSD=4.7%)、良好的稳定性 (两周后拉曼强度仍高于 80%) 和回收率为 96.43%至 112.83%，平均 RSD 为 6.3%。该基底易于使用，无需复杂的样品预处理，因此有望成为食品样品中快速灵敏检测方法的候选方法。
