Jakubek Ryan S, Handen Joseph, White Stephen E, Asher Sanford A, Lednev Igor K
Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA.
Department of Chemistry, University at Albany, SUNY, 1400 Washington Avenue, Albany, New York 12222, USA.
Trends Analyt Chem. 2018 Jun;103:223-229. doi: 10.1016/j.trac.2017.12.002. Epub 2017 Dec 11.
UV resonance Raman (UVRR) spectroscopy is a powerful tool for investigating the structure of biological molecules, such as proteins. Numerous UVRR spectroscopic markers that provide information on the structure and environment of the protein backbone and of amino acid side chains have recently been discovered. Combining these UVRR markers with hydrogen-deuterium exchange and advanced statistics is a powerful tool for studying protein systems, including the structure and formation mechanism of protein aggregates and amyloid fibrils. These techniques allow crucial new insights into the structure and dynamics of proteins, such as polyglutamine peptides, which are associated with 10 different neurodegenerative diseases. Here we summarize the spectroscopic structural markers recently developed and the important insights they provide.
紫外共振拉曼（UVRR）光谱是研究生物分子（如蛋白质）结构的有力工具。最近发现了许多能提供有关蛋白质主链和氨基酸侧链结构及环境信息的UVRR光谱标记物。将这些UVRR标记物与氢-氘交换及先进的统计学方法相结合，是研究蛋白质系统（包括蛋白质聚集体和淀粉样纤维的结构及形成机制）的有力工具。这些技术能让我们对与10种不同神经退行性疾病相关的蛋白质（如多聚谷氨酰胺肽）的结构和动力学有至关重要的新认识。在此，我们总结了最近开发的光谱结构标记物及其提供的重要见解。
