Shahhosseini Elahe, Naddafi Kazem, Nabizadeh Ramin, Shamsipour Mansour, Namvar Zahra, Tayebi Behnoosh, Shoormasti Raheleh Shokouhi, Hassanvand Mohammad Sadegh, Yunesian Masud
1Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
2Center for Air Pollution Research (CAPR), Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran.
J Environ Health Sci Eng. 2019 Aug 11;17(2):789-795. doi: 10.1007/s40201-019-00395-6. eCollection 2019 Dec.
Allergens like endotoxin and mite allergen Der p 1 are associated with early wheezing and asthma morbidity. Day-care centers can be an important source of exposure to allergens. The aim of this study was to evaluate children's exposure to endotoxin and mite allergen (Der p 1) associated with total suspended particulate matter (TSP) and settled dust in day-care centers in two phases in years of 2015 and 2016 in Tehran city, Iran.
Endotoxin and mite allergen Der p 1 in TSP and settled dust were measured in 23 day-care centers in Tehran. After collecting dust samples and weighting them, and then their extraction, Endotoxin and Der p 1 allergen were determined using QCL-1000 Endpoint chromogenic LAL Assay and ELISA, respectively.
The mean concentrations of endotoxin and mite allergen Der p 1 in settled dust were 0.3 EU/mg and 0.2 ng/mg, respectively. The mean concentration of endotoxin and mite allergen Der p 1 in indoor air TSP were 0.8 EU/m and 0.4 ng/m, respectively. A significant negative correlation was found between endotoxin both in settled dust and in TSP with measured relative humidity in winter. Also, moderate correlation was observed between Der p 1 in settled dust and relative humidity in winter; however, the correlation between allergen in TSP and relative humidity was not significant.
Day-care centers can be an important source of endotoxin and Der p 1 allergen, so, implementation of proper interventions in these places can reduce exposure to them.
内毒素和螨过敏原Der p 1等过敏原与早期喘息及哮喘发病相关。日托中心可能是接触过敏原的重要来源。本研究旨在评估2015年和2016年两个阶段伊朗德黑兰市日托中心儿童接触与总悬浮颗粒物（TSP）及沉降灰尘相关的内毒素和螨过敏原（Der p 1）的情况。
对德黑兰23个日托中心的TSP及沉降灰尘中的内毒素和螨过敏原Der p 1进行测量。收集灰尘样本并称重，然后进行提取，分别使用QCL - 1000终点显色鲎试剂法和酶联免疫吸附测定法测定内毒素和Der p 1过敏原。
沉降灰尘中内毒素和螨过敏原Der p 1的平均浓度分别为0.3 EU/mg和0.2 ng/mg。室内空气中TSP内毒素和螨过敏原Der p 1的平均浓度分别为0.8 EU/m和0.4 ng/m。冬季沉降灰尘和TSP中的内毒素与测得的相对湿度之间存在显著负相关。冬季沉降灰尘中的Der p 1与相对湿度之间观察到中度相关性；然而，TSP中过敏原与相对湿度之间的相关性不显著。
日托中心可能是内毒素和Der p 1过敏原的重要来源，因此，在这些场所实施适当干预措施可减少对它们的接触。
