Al-Yousef Hanan M, Fantoukh Omer I, El-Sayed May A, Amina Musarat, Adel Rasha, Hassan Wafaa H B, Abdelaziz Sahar
Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451 Saudi Arabia.
Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, 44519 Zagazig, Egypt.
Saudi J Biol Sci. 2021 Oct;28(10):5609-5616. doi: 10.1016/j.sjbs.2021.05.077. Epub 2021 Jun 4.
The hydroalcoholic extract (MIT) of (Forssk.) growing in Saudi Arabia in addition to the chloroform (MIC) and -butanol (MIB) fractions were investigated for the first time using UPLC-ESI-MS/MS. The analysis revealed the tentative identification of fifty-eight compounds including three organic acids, twenty-five phenolic compounds, three coumarins, two anthocyanins, twenty-one flavonoids, three terpenes, and one miscellaneous. Moreover, the therapeutic potential of (MIT) and its fractions (MIC and MIB) were determined by evaluation of their cytotoxic, antioxidant, and anti-obesity characteristics. The MIT extract showed the highest phenolic (125.23 ± 0.87 mg gallic acid equivalent/100 gm extract) and flavonoid (112.24 ± 2.45 mg quercetin equivalent/100 gm extract) contents followed by -butanol and chloroform fractions. The MIT extract revealed a potent cytotoxic activity against HepG-2 (Hepatocellular carcinoma) and MCF-7 (Breast carcinoma) with IC 28.5 ± 2.0 and 35.2 ± 1.2 µgmL, respectively. Additionally, the tested hydroalcoholic extract exhibited a significant DPPH scavenging activity with SC 28.4 ± 1.2 µg/mL and a remarkable lipase inhibitory activity with IC 54.2 ± 1.2 µg/mL. In conclusion, the current study presents the first insights into the phytochemical constituents and pharmacological properties of extract and its chloroform and -butanol fractions. The results revealed that hydroalcoholic extract might be a prolific source of bioactive constituents with potent antioxidant, cytotoxic and anti-obesity potential. It might be a natural alternative therapy and nutritional strategy for obesity treatment.
首次使用超高效液相色谱-电喷雾串联质谱法（UPLC-ESI-MS/MS）对生长在沙特阿拉伯的（某种植物，原文未给出具体植物名称，用括号代替）的水醇提取物（MIT）以及氯仿（MIC）和正丁醇（MIB）馏分进行了研究。分析结果初步鉴定出58种化合物，包括三种有机酸、25种酚类化合物、三种香豆素、两种花青素、21种黄酮类化合物、三种萜类化合物和一种其他化合物。此外，通过评估其细胞毒性、抗氧化和抗肥胖特性，确定了（某种植物，原文未给出具体植物名称，用括号代替）的提取物（MIT）及其馏分（MIC和MIB）的治疗潜力。MIT提取物的酚类（125.23±0.87毫克没食子酸当量/100克提取物）和黄酮类（112.24±2.45毫克槲皮素当量/100克提取物）含量最高，其次是正丁醇和氯仿馏分。MIT提取物对肝癌细胞HepG-2和乳腺癌细胞MCF-7显示出强大的细胞毒性活性，IC50分别为28.5±2.0和35.2±1.2微克/毫升。此外，受试的水醇提取物表现出显著的二苯基苦味酰基自由基（DPPH）清除活性，半抑制浓度（SC）为28.4±1.2微克/毫升，以及显著的脂肪酶抑制活性，IC50为54.2±1.2微克/毫升。总之，本研究首次深入了解了（某种植物，原文未给出具体植物名称，用括号代替）提取物及其氯仿和正丁醇馏分的植物化学成分和药理特性。结果表明，（某种植物，原文未给出具体植物名称，用括号代替）的水醇提取物可能是生物活性成分的丰富来源，具有强大的抗氧化、细胞毒性和抗肥胖潜力。它可能是肥胖治疗的一种天然替代疗法和营养策略。
