维生素 D3（α-1,25(OH)2D3）可保护视网膜色素上皮免受高氧损伤。
Vitamin-D3 (α-1, 25(OH) 2D3) Protects Retinal Pigment Epithelium From Hyperoxic Insults.
机构信息
,.
出版信息
Invest Ophthalmol Vis Sci. 2020 Feb 7;61(2):4. doi: 10.1167/iovs.61.2.4.
PURPOSE
Oxidative stress affects the retinal pigment epithelium (RPE) leading to development of vascular eye diseases. Cholecalciferol (VIT-D) is a known modulator of oxidative stress and angiogenesis. This in vitro study was carried out to evaluate the protective role of VIT-D on RPE cells incubated under hyperoxic conditions.
METHODS
Cadaver primary RPE (PRPE) cells were cultured in hyperoxia (40% O2) with or without VIT-D (α-1, 25(OH) 2D3). The functional and physiological effects of PRPE cells with VIT-D treatment were analyzed using molecular and biochemical tools.
RESULTS
Vascular signaling modulators, such as vascular endothelial growth factor (VEGF) and Notch, were reduced in hyperoxic conditions but significantly upregulated in the presence of VIT-D. Additionally, PRPE conditioned medium with VIT-D induced the tubulogenesis in primary human umbilical vein endothelial cells (HUVEC) cells. VIT-D supplementation restored phagocytosis and transmembrane potential in PRPE cells cultured under hyperoxia.
CONCLUSIONS
VIT-D protects RPE cells and promotes angiogenesis under hyperoxic insult. These findings may give impetus to the potential of VIT-D as a therapeutic agent in hyperoxia induced retinal vascular diseases.
目的
氧化应激会影响视网膜色素上皮（RPE），从而导致血管性眼病的发生。胆钙化醇（VIT-D）是一种已知的氧化应激和血管生成调节剂。本体外研究旨在评估 VIT-D 在高氧条件下孵育的 RPE 细胞中的保护作用。
方法
培养尸体原代 RPE（PRPE）细胞在高氧（40%O2）环境中，或添加或不添加 VIT-D（α-1, 25(OH)2D3）。使用分子和生化工具分析 VIT-D 处理后 PRPE 细胞的功能和生理效应。
结果
血管信号调节剂，如血管内皮生长因子（VEGF）和 Notch，在高氧条件下减少，但在 VIT-D 存在下显著上调。此外，用 VIT-D 处理的 PRPE 条件培养基可诱导原代人脐静脉内皮细胞（HUVEC）细胞的管形成。VIT-D 补充可恢复高氧培养的 PRPE 细胞的吞噬作用和跨膜电位。
结论
VIT-D 可保护 RPE 细胞并促进高氧损伤下的血管生成。这些发现可能为 VIT-D 作为治疗高氧诱导的视网膜血管疾病的潜在药物提供动力。
Zotero 插件