Sabanci University Nanotechnology Research and Application Center, Istanbul, 34956, Turkey. Sabanci University Faculty of Engineering and Natural Sciences, Istanbul, 34956, Turkey.
Biomed Mater. 2020 Apr 15;15(3):035015. doi: 10.1088/1748-605X/ab7417.
Scaffold-based tissue engineering approaches have been commonly used for skin regeneration or wound healings caused by diseases or trauma. For an ideal complete healing process, scaffold structures need to meet the criteria of biocompatibility, biodegradability, and antimicrobial properties, as well as to provide geometrical necessities for the regeneration of damaged tissue. In this study, design, synthesis and characterization of a three dimensional (3D) printable copolymer based on polycaprolactone-block-poly(1,3-propylene succinate) (PCL-PPSu) including anti-microbial silver particles is presented. 3D printing of PCL-PPSu copolymers provided a lower processing temperature compared to neat PCL, hence, inclusion of temperature-sensitive bioactive reagents into the developed copolymer could be realized. In addition, 3D printed block copolymer showed an enhanced hydrolytic and enzymatic degradation behavior. Cell viability and cytotoxicity of the developed copolymer were evaluated by using human dermal fibroblast (HDF) cells. The addition of silver nitrate within the polymer matrix resulted in a significant decrease in the adhesion of different types of microorganisms on the scaffold without inducing any cytotoxicity on HDF cells in vitro. The results suggested that 3D printed PCL-PPSu scaffolds containing anti-microbial silver particles could be considered as a promising biomaterial for emerging skin regenerative therapies, in the light of its adaptability to 3D printing technology, low-processing temperature, enhanced degradation behavior and antimicrobial properties.
支架组织工程方法已广泛用于因疾病或创伤引起的皮肤再生或伤口愈合。为了实现理想的完全愈合过程，支架结构需要满足生物相容性、可生物降解性和抗菌性能的标准，并为受损组织的再生提供几何必要性。在这项研究中，设计、合成和表征了一种基于聚己内酯嵌段-聚（1,3-丙交酯）（PCL-PPSu）的三维（3D）可打印共聚物，其中包括抗菌银颗粒。与纯 PCL 相比，PCL-PPSu 共聚物的 3D 打印提供了更低的加工温度，因此可以将温度敏感的生物活性试剂纳入开发的共聚物中。此外，3D 打印的嵌段共聚物表现出增强的水解和酶降解行为。通过使用人真皮成纤维细胞（HDF）细胞评估了开发的共聚物的细胞活力和细胞毒性。在聚合物基质中添加硝酸银导致不同类型的微生物在支架上的粘附显著减少，而在体外对 HDF 细胞没有诱导任何细胞毒性。结果表明，含有抗菌银颗粒的 3D 打印 PCL-PPSu 支架可以被认为是一种有前途的新兴皮肤再生治疗的生物材料，因为其适应 3D 打印技术、低加工温度、增强的降解行为和抗菌性能。
