Yin Zhengqiao, Zhang Shengmiao, Liu Xiucai
Shanghai Key Laboratory of Advanced Polymeric Materials, Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.
Polymers (Basel). 2023 Apr 13;15(8):1862. doi: 10.3390/polym15081862.
The immobilized cell fermentation technique (IMCF) has gained immense popularity in recent years due to its capacity to enhance metabolic efficiency, cell stability, and product separation during fermentation. Porous carriers used as cell immobilization facilitate mass transfer and isolate the cells from an adverse external environment, thus accelerating cell growth and metabolism. However, creating a cell-immobilized porous carrier that guarantees both mechanical strength and cell stability remains challenging. Herein, templated by water-in-oil (w/o) high internal phase emulsions (HIPE), we established a tunable open-cell polymeric P(St--GMA) monolith as a scaffold for the efficient immobilization of (. The porous framework's mechanical property was substantially improved by incorporating the styrene monomer and cross-linker divinylbenzene (DVB) in the HIPE's external phase, while the epoxy groups on glycidyl methacrylate (GMA) supply anchoring sites for , securing the immobilization to the inner wall surface of the void. For the fermentation of immobilized , the polyHIPEs permit efficient mass transfer, which increases along with increased interconnectivity of the monolith, resulting in higher -lactic acid yield compared to that of suspended cells with an increase of 17%. The relative -lactic acid production is constantly maintained above 92.9% of their initial relative production after 10 cycles, exhibiting both its great cycling stability and the durability of the material structure. Furthermore, the procedure during recycle batch also simplifies downstream separation operations.
近年来，固定化细胞发酵技术（IMCF）因其在发酵过程中提高代谢效率、细胞稳定性和产物分离能力而广受欢迎。用作细胞固定化的多孔载体促进了传质，并将细胞与不利的外部环境隔离开来，从而加速了细胞生长和代谢。然而，制造一种既能保证机械强度又能保证细胞稳定性的固定化细胞多孔载体仍然具有挑战性。在此，以油包水（w/o）高内相乳液（HIPE）为模板，我们建立了一种可调谐的开孔聚合物P（St-GMA）整体材料作为有效固定化（此处原文缺失具体物质）的支架。通过在HIPE的外相中加入苯乙烯单体和交联剂二乙烯基苯（DVB），多孔框架的机械性能得到了显著改善，而甲基丙烯酸缩水甘油酯（GMA）上的环氧基团为（此处原文缺失具体物质）提供了锚固位点，确保其固定在孔隙的内壁表面。对于固定化（此处原文缺失具体物质）的发酵，聚HIPE允许高效传质，传质随着整体材料互连性的增加而增加，与悬浮细胞相比，-乳酸产量提高了17%。在10个循环后，相对-乳酸产量始终保持在其初始相对产量的92.9%以上，既显示出其良好的循环稳定性，又显示出材料结构的耐久性。此外，循环批次过程还简化了下游分离操作。
