Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, United States.
Department of Biostatistics and Medical Informatics, University of Wisconsin-Madison, Madison, WI, United States.
Front Immunol. 2020 Jan 24;10:3065. doi: 10.3389/fimmu.2019.03065. eCollection 2019.
A successful pregnancy requires many physiological adaptations from the mother, including the establishment of tolerance toward the semiallogeneic fetus. Innate lymphoid cells (ILCs) have arisen as important players in immune regulation and tissue homeostasis at mucosal and barrier surfaces. Dimensionality reduction and transcriptomic analysis revealed the presence of two novel CD56 decidual ILCs that express low T-bet and divergent Eomes levels. Transcriptional correlation with recently identified first trimester decidual dNKs suggests that these novel decidual ILCs might be present throughout pregnancy. Functional testing with permutation analysis revealed production of multiple factors by individual cells, with a preference for IFNγ and VEGF. Overall, our data suggests continuity of a unique decidual innate lymphocytes across pregnancy with a polyfunctional functional profile conducive for pregnancy.
成功的妊娠需要母体做出许多生理上的适应，包括对半同种异体胎儿建立耐受。先天淋巴细胞（ILCs）已成为黏膜和屏障表面免疫调节和组织稳态的重要参与者。降维和转录组分析显示存在两种新型 CD56 蜕膜 ILC，其表达低 T-bet 和不同的 Eomes 水平。与最近鉴定的早孕蜕膜 dNK 转录相关性表明，这些新型蜕膜 ILC 可能存在于整个妊娠期间。通过置换分析的功能测试显示单个细胞产生多种因子，偏爱 IFNγ 和 VEGF。总体而言，我们的数据表明独特的妊娠蜕膜固有淋巴细胞具有跨妊娠的连续性和有利于妊娠的多功能功能特征。
