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由九头蛇刺细胞蛋白微胶原蛋白-1制成的二硫键交联水凝胶。

Disulfide Crosslinked Hydrogels Made From the Hydra Stinging Cell Protein, Minicollagen-1.

作者信息

Farajollahi Sanaz, Dennis Patrick B, Crosby Marquise G, Slocik Joseph M, Pelton Anthony T, Hampton Cheri M, Drummy Lawrence F, Yang Steven J, Silberstein Meredith N, Gupta Maneesh K, Naik Rajesh R

机构信息

Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, Dayton, OH, United States.

UES Inc., Dayton, OH, United States.

出版信息

Front Chem. 2020 Jan 23;7:950. doi: 10.3389/fchem.2019.00950. eCollection 2019.

DOI:10.3389/fchem.2019.00950
PMID:32039158
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6989532/
Abstract

Minicollagens from cnidarian nematocysts are attractive potential building blocks for the creation of strong, lightweight and tough polymeric materials with the potential for dynamic and reconfigurable crosslinking to modulate functionality. In this study, the minicollagen-1 isoform was recombinantly expressed in bacteria, and a high throughput purification protocol was developed to generate milligram levels of pure protein without column chromatography. The resulting minicollagen-1 preparation demonstrated spectral properties similar to those observed with collagen and polyproline sequences as well as the ability to self-assemble into oriented fibers and bundles. Photo-crosslinking with Ru(II) was used to create robust hydrogels that were analyzed by mechanical testing. Interestingly, the minicollagen-1 hydrogels could be dissolved with reducing agents, indicating that ruthenium-mediated photo-crosslinking was able to induce disulfide metathesis to create the hydrogels. Together, this work is an important first step in creating minicollagen-based materials whose properties can be manipulated through static and reconfigurable post-translational modifications.

摘要

来自刺胞动物刺丝囊的微胶原蛋白是极具吸引力的潜在构建模块，可用于制造坚固、轻质且坚韧的聚合材料，具有动态和可重构交联以调节功能的潜力。在本研究中，微胶原蛋白-1亚型在细菌中重组表达，并开发了一种高通量纯化方案，无需柱色谱即可产生毫克级的纯蛋白。所得的微胶原蛋白-1制剂表现出与胶原蛋白和多聚脯氨酸序列相似的光谱特性，以及自组装成定向纤维和束的能力。使用钌（II）进行光交联以创建坚固的水凝胶，并通过力学测试进行分析。有趣的是，微胶原蛋白-1水凝胶可以用还原剂溶解，这表明钌介导的光交联能够诱导二硫键复分解以形成水凝胶。总之，这项工作是创建基于微胶原蛋白的材料的重要第一步，其性质可通过静态和可重构的翻译后修饰来操纵。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7318/6989532/436926a441b5/fchem-07-00950-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7318/6989532/e0a7b127bc82/fchem-07-00950-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7318/6989532/85f77c013575/fchem-07-00950-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7318/6989532/57ca86f53aa3/fchem-07-00950-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7318/6989532/5ff2e532381b/fchem-07-00950-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7318/6989532/534e7f42c82a/fchem-07-00950-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7318/6989532/94123a36f65e/fchem-07-00950-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7318/6989532/436926a441b5/fchem-07-00950-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7318/6989532/e0a7b127bc82/fchem-07-00950-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7318/6989532/85f77c013575/fchem-07-00950-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7318/6989532/57ca86f53aa3/fchem-07-00950-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7318/6989532/5ff2e532381b/fchem-07-00950-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7318/6989532/534e7f42c82a/fchem-07-00950-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7318/6989532/94123a36f65e/fchem-07-00950-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7318/6989532/436926a441b5/fchem-07-00950-g0007.jpg

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