Department of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut, Lebanon.
Department of Cell and Molecular Biology, Team Cytokines and Nitric oxide synthases, Faculty of Biology, University of Sciences and Technology Houari Boumediene USTHB, Algiers, Algeria.
PLoS One. 2020 Feb 10;15(2):e0227928. doi: 10.1371/journal.pone.0227928. eCollection 2020.
Breast cancer is one of the commonest cancers among Algerian females. Compared to Western populations, the median age of diagnosis of breast cancer is much lower in Algeria. The objective of this study is to explore the expression of several miRNAs reported to be deregulated in breast cancer. The miRNAs miR-21, miR-125b, miR-100, miR-425-5p, miR-200c, miR-183 and miR-182 were studied on tumor and normal adjacent Algerian breast tissues using quantitative reverse transcription real time PCR, and the results were analyzed according to clinical characteristics. Compared to the normal adjacent tissues, miR-21, miR-183, miR-182, miR-425-5p and miR-200c were found to be upregulated while miR-100 and miR-125b were insignificantly deregulated. A positive correlation was noted among miR-183, miR-182 and miR-200c and among miR-425-5p, miR-183, miR-200c and miR-21. Further global miRNA microarray profiling studies can aid in finding ethnic specific miRNA biomarkers in the Algerian breast cancer population.
乳腺癌是阿尔及利亚女性中最常见的癌症之一。与西方人群相比，阿尔及利亚乳腺癌的中位诊断年龄要低得多。本研究旨在探讨几种已报道在乳腺癌中失调的 miRNAs 的表达情况。使用定量逆转录实时 PCR 研究了肿瘤和正常相邻的阿尔及利亚乳腺组织中的 miR-21、miR-125b、miR-100、miR-425-5p、miR-200c、miR-183 和 miR-182，根据临床特征对结果进行了分析。与正常相邻组织相比，发现 miR-21、miR-183、miR-182、miR-425-5p 和 miR-200c 上调，而 miR-100 和 miR-125b 则无明显失调。miR-183、miR-182 和 miR-200c 之间以及 miR-425-5p、miR-183、miR-200c 和 miR-21 之间存在正相关。进一步的全局 miRNA 微阵列分析研究可以帮助发现阿尔及利亚乳腺癌人群中具有种族特异性的 miRNA 生物标志物。
