Department of Geriatrics, Donald W. Reynolds Institute on Aging, Center for Translational Research in Aging & Longevity, University of Arkansas for Medical Sciences, 4301 West Markham Street, Slot 806, Little Rock, AR, 72205-7199, USA.
Department of Geriatrics, Donald W. Reynolds Institute on Aging, University of Arkansas for Medical Sciences, 4301 W Markham Street, Slot 748, Little Rock, AR, 72205-7199, USA.
J Int Soc Sports Nutr. 2020 Feb 10;17(1):9. doi: 10.1186/s12970-020-0340-5.
We have determined the acute response of protein kinetics to one or two servings (6.3 g and 12.6 g) of a proprietary composition containing free-form essential amino acids (EAA) (3.2 g EAA per serving) and whey protein (2.4 g per serving), as well as the response to consumption of a popular whey-based protein supplement (Gatorade Recover) (17 g; 12.6 g protein).
Whole-body rates of protein synthesis, breakdown and net balance (taken to be the anabolic response) were determined using primed-constant infusions of H-phenylalnine and H-tyrosine. Muscle protein fractional synthetic rate (FSR) was also determined with the H-phenylalanine tracer.
Plasma EAA levels increased following consumption of all beverages, with the greatest response in the high-dose EAA/protein composition. Similarly, the increase in net balance between whole-body protein synthesis and breakdown was greatest following consumption of the high-dose EAA/protein composition, while the low-dose EAA/protein composition and Gatorade Recover induced similar increases in net balance. When the net balance response was normalized for the total amount of product given, the high- and low-dose EAA/protein beverages were approximately 6- and 3-fold more anabolic than the Gatorade Recover, respectively. The greater anabolic response to the EAA/protein composition was due to greater increases in whole-body protein synthesis with both doses, and a markedly greater suppression of whole-body protein breakdown in the high-dose group. Muscle protein FSR after beverage consumption reflected changes in whole-body protein synthesis, with the larger EAA/protein dose significantly increasing FSR.
We conclude that a composition of a balanced EAA formulation combined with whey protein is highly anabolic as compared to a whey protein-based recovery product, and that the response is dose-dependent.
ClinicalTrials.gov Identifier: NCT03502941. This trial was registered on April 19, 2018.
我们已经确定了蛋白质动力学对一种或两种特定配方（每份含有 6.3 克和 12.6 克游离形式必需氨基酸（EAA）和乳清蛋白（每份 2.4 克）的急性反应）以及消费流行的乳清蛋白补充剂（佳得乐恢复）（17 克；12.6 克蛋白质）的反应。
使用 H-苯丙氨酸和 H-酪氨酸的脉冲恒速输注来确定全身蛋白质合成、分解和净平衡（被认为是合成代谢反应）的速率。肌肉蛋白质分数合成率（FSR）也使用 H-苯丙氨酸示踪剂进行测定。
所有饮料饮用后，血浆 EAA 水平升高，高剂量 EAA/蛋白质配方反应最大。同样，高剂量 EAA/蛋白质配方的全身蛋白质合成与分解之间的净平衡增加最大，而低剂量 EAA/蛋白质配方和佳得乐恢复则引起类似的净平衡增加。当将净平衡反应归一化为给予的产品总量时，高剂量和低剂量 EAA/蛋白质饮料的合成代谢作用分别比佳得乐恢复高约 6 倍和 3 倍。EAA/蛋白质配方的更大合成代谢反应归因于两种剂量下全身蛋白质合成的更大增加，以及高剂量组全身蛋白质分解的明显抑制。饮料消耗后的肌肉蛋白质 FSR 反映了全身蛋白质合成的变化，较大的 EAA/蛋白质剂量显著增加了 FSR。
我们得出结论，与基于乳清蛋白的恢复产品相比，平衡的 EAA 配方与乳清蛋白的组合具有高度的合成代谢作用，并且反应是剂量依赖性的。
ClinicalTrials.gov 标识符：NCT03502941。该试验于 2018 年 4 月 19 日注册。
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