Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Mobile Healthcare Laboratory, Device and System Research Center, Samsung Advanced Institute of Technology, Samsung Electronics Co. Ltd., 130 Samsung-ro Yeongtong-gu, Suwon-si, Gyeonggi-do 16678, Republic of Korea.
Sci Adv. 2020 Jan 24;6(4):eaay5206. doi: 10.1126/sciadv.aay5206. eCollection 2020 Jan.
Noninvasive blood glucose monitoring has been a long-standing dream in diabetes management. The use of Raman spectroscopy, with its molecular specificity, has been investigated in this regard over the past decade. Previous studies reported on glucose sensing based on indirect evidence such as statistical correlation to the reference glucose concentration. However, these claims fail to demonstrate glucose Raman peaks, which has raised questions regarding the effectiveness of Raman spectroscopy for glucose sensing. Here, we demonstrate the first direct observation of glucose Raman peaks from in vivo skin. The signal intensities varied proportional to the reference glucose concentrations in three live swine glucose clamping experiments. Tracking spectral intensity based on linearity enabled accurate prospective prediction in within-subject and intersubject models. Our direct demonstration of glucose signal may quiet the long debate about whether glucose Raman spectra can be measured in vivo in transcutaneous glucose sensing.
非侵入式血糖监测一直是糖尿病管理领域的一个长期梦想。在过去的十年中，拉曼光谱以其分子特异性，在这方面得到了研究。过去的研究报告了基于与参考葡萄糖浓度的统计相关性等间接证据的葡萄糖传感。然而，这些说法未能证明葡萄糖拉曼峰的存在，这引发了人们对拉曼光谱用于葡萄糖传感的有效性的质疑。在这里，我们首次直接观察到了来自活体皮肤的葡萄糖拉曼峰。在三个活体猪血糖钳夹实验中，信号强度与参考葡萄糖浓度呈比例变化。基于线性的光谱强度跟踪能够实现受试者内和受试者间模型的准确前瞻性预测。我们对葡萄糖信号的直接演示可能会平息关于在经皮血糖传感中是否可以在体内测量葡萄糖拉曼光谱的长期争论。
