Artigas-Jerónimo Sara, Comín Juan J Pastor, Villar Margarita, Contreras Marinela, Alberdi Pilar, Viera Israel León, Soto Leandro, Cordero Raúl, Valdés James J, Cabezas-Cruz Alejandro, Estrada-Peña Agustín, Fuente José de la
SaBio. Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain.
Centro de Investigación y Documentación Musical CIDoM-UCLM-CSIC, Facultad de Educación de Ciudad Real, Ronda Calatrava 3, 13071 Ciudad Real, Spain.
Vaccines (Basel). 2020 Feb 8;8(1):77. doi: 10.3390/vaccines8010077.
The main objective of this study was to propose a novel methodology to approach challenges in molecular biology. Akirin/Subolesin (AKR/SUB) are vaccine protective antigens and are a model for the study of the interactome due to its conserved function in the regulation of different biological processes such as immunity and development throughout the metazoan. Herein, three visual artists and a music professor collaborated with scientists for the functional characterization of the AKR2 interactome in the regulation of the NF-κB pathway in human placenta cells. The results served as a methodological proof-of-concept to advance this research area. The results showed new perspectives on unexplored characteristics of AKR2 with functional implications. These results included protein dimerization, the physical interactions with different proteins simultaneously to regulate various biological processes defined by cell type-specific AKR-protein interactions, and how these interactions positively or negatively regulate the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway in a biological context-dependent manner. These results suggested that AKR2-interacting proteins might constitute suitable secondary transcription factors for cell- and stimulus-specific regulation of NF-κB. Musical perspective supported AKR/SUB evolutionary conservation in different species and provided new mechanistic insights into the AKR2 interactome. The combined scientific and artistic perspectives resulted in a multidisciplinary approach, advancing our knowledge on AKR/SUB interactome, and provided new insights into the function of AKR2-protein interactions in the regulation of the NF-κB pathway. Additionally, herein we proposed an algorithm for quantum vaccinomics by focusing on the model proteins AKR/SUB.
本研究的主要目的是提出一种新方法来应对分子生物学中的挑战。Akirin/Subolesin（AKR/SUB）是疫苗保护性抗原，由于其在调节后生动物不同生物过程（如免疫和发育）中的保守功能，是研究相互作用组的一个模型。在此，三位视觉艺术家和一位音乐教授与科学家合作，对人胎盘细胞中AKR2相互作用组在NF-κB信号通路调节中的功能进行了表征。这些结果作为一种方法学概念验证，推动了这一研究领域的发展。结果显示了AKR2未被探索的特征的新视角，这些特征具有功能意义。这些结果包括蛋白质二聚化、与不同蛋白质同时发生物理相互作用以调节由细胞类型特异性AKR-蛋白质相互作用定义的各种生物过程，以及这些相互作用如何以生物学背景依赖的方式正向或负向调节活化B细胞的核因子κB轻链增强子（NF-κB）信号通路。这些结果表明，与AKR2相互作用的蛋白质可能构成适合对NF-κB进行细胞和刺激特异性调节的二级转录因子。音乐视角支持了AKR/SUB在不同物种中的进化保守性，并为AKR2相互作用组提供了新的机制见解。科学与艺术视角的结合产生了一种多学科方法，增进了我们对AKR/SUB相互作用组的了解，并为AKR2-蛋白质相互作用在NF-κB信号通路调节中的功能提供了新的见解。此外，在此我们通过聚焦模型蛋白AKR/SUB提出了一种量子疫苗组学算法。
