板蓝根多糖对猪睾丸细胞中伪狂犬病病毒的抗病毒活性。
Antiviral activities of Radix isatidis polysaccharide against pseudorabies virus in swine testicle cells.
机构信息
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450000, China.
Wuhu Overseas Student Pioneer Park, Wuhu, 241006, China.
出版信息
BMC Complement Med Ther. 2020 Feb 11;20(1):48. doi: 10.1186/s12906-020-2838-4.
BACKGROUND
Radix isatidis has been used in China and other Asian countries for its antiviral and anti-inflammatory effects for thousands of years. However, the antiviral effect of Radix isatidis polysaccharide against pseudorabies virus (PRV) is still unknown.
METHODS
The polysaccharide were isolated from extract of the roots of Radix isatidis. MTT assays were used to determine the preventive effect, inhibitory effect and antiviral effect of Radix isatidis polysaccharide on PRV in vitro.
RESULTS
This study found that different concentrations of polysaccharides from this plant can inhibit PRV replication by 14.674-30.840%, prevent infection at rates of 6.668-14.923%, and kill this virus at rates of 32.214-67.422%.
CONCLUSION
These results broaden the understanding of this traditional Chinese herb and provide a theoretical basis for further research. Moreover, Radix isatidis polysaccharide could be used for antiviral therapy.
背景
板蓝根在我国及亚洲其他国家已有数千年的应用历史，具有抗病毒和抗炎作用。然而，板蓝根多糖抗猪伪狂犬病病毒（PRV）的作用尚不清楚。
方法
从板蓝根根提取物中分离多糖。MTT 法测定板蓝根多糖对 PRV 的体外预防作用、抑制作用和抗病毒作用。
结果
本研究发现，不同浓度的该植物多糖可抑制 PRV 复制，抑制率为 14.674%-30.840%；预防感染率为 6.668%-14.923%；杀灭病毒率为 32.214%-67.422%。
结论
这些结果拓宽了对这种传统中药的认识，为进一步研究提供了理论依据。此外，板蓝根多糖可用于抗病毒治疗。
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