Sharma Shivani, Kaur Gazaldeep, Kumar Anil, Meena Varsha, Ram Hasthi, Kaur Jaspreet, Pandey Ajay Kumar
Department of Biotechnology, National Agri-Food Biotechnology Institute, Sector 81, Knowledge City, Mohali, Punjab 140306, India.
University Institute of Engineering and Technology, Sector 25, Panjab University, Chandigarh, Punjab 160015, India.
Plants (Basel). 2020 Feb 11;9(2):229. doi: 10.3390/plants9020229.
Iron is one of the important micronutrients that is required for crop productivity and yield-related traits. To address the Fe homeostasis in crop plants, multiple transporters belonging to the category of major facilitator superfamily are being explored. In this direction, earlier vacuolar iron transporters (VITs) have been reported and characterized functionally to address biofortification in cereal crops. In the present study, the identification and characterization of new members of vacuolar iron transporter-like proteins (VTL) was performed in wheat. Phylogenetic distribution demonstrated distinct clustering of the identified genes from the previously known genes. Our analysis identifies multiple genes from hexaploid wheat with the highest number genes localized on chromosome 2. Quantitative expression analysis suggests that most of the genes are induced mostly during the Fe surplus condition, thereby reinforcing their role in metal homeostasis. Interestingly, most of the wheat genes were also significantly up-regulated in a tissue-specific manner under Zn, Mn and Cu deficiency. Although, no significant changes in expression of wheat genes were observed in roots under heavy metals, but , and were upregulated in the presence of cobalt stress. Overall, this work deals with the detailed characterization of wheat genes that could provide an important genetic framework for addressing metal homeostasis in bread wheat.
铁是作物生产力和产量相关性状所需的重要微量营养素之一。为了解决作物中铁的稳态问题，人们正在探索多种属于主要转运体超家族的转运蛋白。在这方面，早期已报道了液泡铁转运蛋白（VITs）并对其功能进行了表征，以解决谷类作物的生物强化问题。在本研究中，对小麦中液泡铁转运蛋白样蛋白（VTL）新成员进行了鉴定和表征。系统发育分布表明，所鉴定的基因与先前已知的基因有明显的聚类。我们的分析从小麦六倍体中鉴定出多个基因，其中数量最多的基因位于2号染色体上。定量表达分析表明，大多数基因主要在铁过剩条件下被诱导，从而加强了它们在金属稳态中的作用。有趣的是，在锌、锰和铜缺乏的情况下，大多数小麦基因也以组织特异性方式显著上调。虽然在重金属处理下，小麦基因在根中的表达没有显著变化，但在钴胁迫下，基因、和被上调。总体而言，这项工作对小麦基因进行了详细表征，可为解决面包小麦中的金属稳态提供重要的遗传框架。
