Cheng Yeong-Hsiang, Hsieh Yi-Chun, Yu Yu-Hsiang
Department of Biotechnology and Animal Science, National Ilan University, Yilan, Taiwan.
J Poult Sci. 2019 Apr 25;56(2):128-139. doi: 10.2141/jpsa.0180067.
is a well-known Chinese medicinal fungus that has been used as a nutraceutical food in several Asian countries. Cordycepin (3'-deoxyadenosine), a secondary metabolite produced from , has been demonstrated to exert a wide spectrum of pharmacological activities, such as anti-microbial and antitumor activities. However, the effect of cordycepin on immunomodulation in broilers is poorly investigated. In the current study, we investigated the effect of cordycepin (9.69, 19.38, and 38.76 mg) from hot water extract (CMHW) on growth performance and immunocompetence in broilers. Results showed that CMHW significantly decreased inducible nitric oxide synthase (iNOS) mRNA levels in the bursa of Fabricius after 4 weeks of feeding (<0.05). CMHW treatment reduced cyclooxygenase-2 (COX-2) mRNA levels in the spleen and bursa of Fabricius after 4 weeks of feeding (<0.05). Supplementation of CMHW for 3 days after vaccination reduced iNOS mRNA level in the spleen of 14 and 28 day-old broilers (<0.05). Prior to vaccination, CMHW pretreatment significantly down-regulated COX-2 mRNA levels in the spleen and bursa of Fabricius of 14-day-old broilers (<0.05). Furthermore, CMHW significantly reduced lipopolysaccharide (LPS)-induced iNOS and COX-2 mRNA levels in the spleen and bursa of Fabricius (<0.05). CMHW treatment attenuated LPS-induced IFN- expression in the spleen and bursa of Fabricius, whereas CMHW induced IL-4 expression in these organs in response to LPS challenge (<0.05). Taken together, these observations demonstrate that CMHW exerts an immunomodulatory role in broilers. CMHW is a potential novel feed additive with applications in inflammation-related diseases and bacterial infection in broilers.
是一种著名的中国药用真菌，在几个亚洲国家被用作营养食品。虫草素（3'-脱氧腺苷）是由 产生的次生代谢产物，已被证明具有广泛的药理活性，如抗菌和抗肿瘤活性。然而，虫草素对肉鸡免疫调节的作用研究较少。在本研究中，我们研究了热水提取物（CMHW）中的虫草素（9.69、19.38和38.76毫克）对肉鸡生长性能和免疫能力的影响。结果表明，喂食4周后，CMHW显著降低了法氏囊中诱导型一氧化氮合酶（iNOS）的mRNA水平（<0.05）。喂食4周后，CMHW处理降低了脾脏和法氏囊中环氧合酶-2（COX-2）的mRNA水平（<0.05）。接种疫苗后补充CMHW 3天可降低14日龄和28日龄肉鸡脾脏中iNOS的mRNA水平（<0.05）。在接种疫苗前，CMHW预处理显著下调了14日龄肉鸡脾脏和法氏囊中COX-2的mRNA水平（<0.05）。此外，CMHW显著降低了脂多糖（LPS）诱导的脾脏和法氏囊中iNOS和COX-2的mRNA水平（<0.05）。CMHW处理减弱了LPS诱导的脾脏和法氏囊中IFN-的表达，而CMHW在LPS刺激下诱导了这些器官中IL-4的表达（<0.05）。综上所述，这些观察结果表明CMHW在肉鸡中发挥免疫调节作用。CMHW是一种潜在的新型饲料添加剂，可用于肉鸡炎症相关疾病和细菌感染。
