在大鼠模型中使用合成磷酸八钙联合骨基质明胶重建下颌骨缺损
Reconstruction of mandibular defects using synthetic octacalcium phosphate combined with bone matrix gelatin in rat model.
作者信息
Sargolzaei-Aval Fereydoon, Saberi Eshagh Ali, Arab Mohammad Reza, Sargolzaei Narjes, Zare Esmaeel, Shahraki Heshmatollah, Sanchooli Tayebeh, Sargolzaeiaval Forough, Arab Maryam
机构信息
Department of Anatomical Sciences, Cellular and Molecular Research Center, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Department of Endodontics, School of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran.
出版信息
Dent Res J (Isfahan). 2020 Jan 21;17(1):10-18. eCollection 2020 Jan-Feb.
BACKGROUND
Regeneration of bone defects remains a challenge for maxillofacial surgeons. The objective of this study was to assess the osteogenic potential of octacalcium phosphate (OCP) and bone matrix gelatin (BMG) alone and in combination with together in artificially created mandibular bone defects.
MATERIALS AND METHODS
In this experimental study Forty-eight male Sprague-Dawley rats (6-8 weeks old) were randomly divided into four groups. Defects were created in the mandible of rats and filled with 10 mg of OCP, BMG, or a combination of both (1/4 ratio). Defects were left unfilled in the control group. To assess bone regeneration and determine the amount of the newly formed bone, specimens were harvested at 7, 14, 21, and 56 days postimplantation. The specimens were processed routinely and studied histologically and histomorphometrically using the light microscope and eyepiece graticule. The amount of newly formed bone was quantitatively measured using histomorphometric methods. Histomorphometric data were analyzed using SPSS software. Mean, standard deviation, mode, and medians were calculated. Tukey HSD test was used to compare the means in all groups. < 0.05 was considered as statistically significant (i.e., 5% significant level).
RESULTS
In the experimental groups, the new bone formation was initiated from the margin of defects during the 7-14 days after implantation. By the end of study, the amount of newly formed bone increased and relatively matured, and almost all of the implanted materials were absorbed. In the control group, slight amount of new bone had been formed at the defect margins (next to the host bone) on day 56. The histomorphometric analysis revealed statistically significant differences in the amount of newly formed bone between the experimental and the control groups ( < 0.001).
CONCLUSION
Combination of OCP/BMG may serve as an optimal biomaterial for the treatment of mandibular bone defects.
背景
骨缺损的修复仍然是颌面外科医生面临的一项挑战。本研究的目的是评估单独使用磷酸八钙（OCP）和骨基质明胶（BMG）以及两者联合使用对人工制造的下颌骨缺损的成骨潜力。
材料与方法
在本实验研究中，48只6 - 8周龄的雄性Sprague-Dawley大鼠被随机分为四组。在大鼠下颌骨制造缺损，并分别填充10毫克OCP、BMG或两者的组合（比例为1/4）。对照组缺损不填充。为评估骨再生并确定新形成骨的量，在植入后7、14、21和56天采集标本。标本常规处理，使用光学显微镜和目镜测微尺进行组织学和组织形态计量学研究。使用组织形态计量学方法对新形成骨的量进行定量测量。组织形态计量学数据使用SPSS软件进行分析。计算均值、标准差、众数和中位数。采用Tukey HSD检验比较所有组的均值。P < 0.05被认为具有统计学意义（即5%显著水平）。
结果
在实验组中，植入后7 - 14天新骨形成从缺损边缘开始。到研究结束时，新形成骨的量增加且相对成熟，几乎所有植入材料均被吸收。在对照组中，56天时在缺损边缘（靠近宿主骨处）形成了少量新骨。组织形态计量学分析显示实验组和对照组新形成骨的量存在统计学显著差异（P < 0.001）。
结论
OCP/BMG组合可能是治疗下颌骨缺损的最佳生物材料。
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