关于寡核苷酸引发的RNA合成机制。II. 适用于DNA序列分析的特异性引物引发的RNA拷贝的合成。
On the mechanism of oligonucleotide-primed RNA synthesis. II. Synthesis of specific primer-initiated RNA copies suitable for DNA sequence analysis.
作者信息
Van Kreijl C F, Beelen R H, Borst P
出版信息
Nucleic Acids Res. 1977 Feb;4(2):445-55. doi: 10.1093/nar/4.2.445.
Abstract
The effect of temperature and primer concentration on oligonucleotide-primed transcription has been studied using the separated strands of a well-defined natural DNA as template. Results were similar to those obtained in the homopolymer-directed model systems. At high temperature and excess primer concentration mainly primer-initiated RNA copies are synthesized. Omission of one ribonucleoside triphosphate also makes the termination specific. The unique RNA fragments thus obtained have been used to determine the perfectly-repeated sequence of 68 base pairs in this DNA.
摘要
利用一条明确的天然DNA的单链作为模板，研究了温度和引物浓度对寡核苷酸引发转录的影响。结果与在均聚物导向模型系统中获得的结果相似。在高温和过量引物浓度下，主要合成引物起始的RNA拷贝。省略一种核糖核苷三磷酸也会使终止具有特异性。由此获得的独特RNA片段已被用于确定该DNA中68个碱基对的完全重复序列。
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本文引用的文献
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2
Nucleotide sequence of a ribonucleic acid transcribed in vitro from lambda phage deoxyribonucleic acid.从λ噬菌体脱氧核糖核酸体外转录的核糖核酸的核苷酸序列。
J Biol Chem. 1971 Aug 25;246(16):5120-39.
3
Transcription of the early region of bacteriophage T7: selective initiation with dinucleotides.噬菌体T7早期区域的转录：二核苷酸的选择性起始
J Mol Biol. 1973 Jun 25;77(2):255-77. doi: 10.1016/0022-2836(73)90335-5.
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The nucleotide sequence of the lactose messenger ribonucleic acid transcribed from the UV5 promoter mutant of Escherichia coli.从大肠杆菌UV5启动子突变体转录而来的乳糖信使核糖核酸的核苷酸序列。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3585-9. doi: 10.1073/pnas.70.12.3585.
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The nucleotide sequence of the lac operator.乳糖操纵基因的核苷酸序列。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3581-4. doi: 10.1073/pnas.70.12.3581.
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Nature of the base sequence conserved in the mitochondrial DNA of a low-density petite.低密度小菌落线粒体DNA中保守的碱基序列的性质
Biochim Biophys Acta. 1973 Jul 13;312(3):441-57. doi: 10.1016/0005-2787(73)90443-7.
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