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一种对克隆的雌激素受体能做出正确反应的新型核转录系统。

A novel nuclear transcription system which responds correctly to cloned estrogen receptor.

作者信息

Thorburn A, Knowland J

机构信息

Department of Biochemistry, Oxford, UK.

出版信息

Nucleic Acids Res. 1988 Nov 25;16(22):10469-76. doi: 10.1093/nar/16.22.10469.

DOI:10.1093/nar/16.22.10469
PMID:3205714
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC338918/
Abstract

We describe here a novel but simple nuclear transcription system in which nuclei make RNA in an isotonic buffer for a long time and respond to a cloned external factor by accurately initiating new transcription.

摘要

我们在此描述一种新颖但简单的核转录系统,在该系统中,细胞核可在等渗缓冲液中长时间合成RNA,并通过准确启动新的转录来响应克隆的外部因子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d68/338918/b62ce4be40de/nar00164-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d68/338918/053b5426d972/nar00164-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d68/338918/b62ce4be40de/nar00164-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d68/338918/053b5426d972/nar00164-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d68/338918/b62ce4be40de/nar00164-0074-a.jpg

相似文献

1
A novel nuclear transcription system which responds correctly to cloned estrogen receptor.一种对克隆的雌激素受体能做出正确反应的新型核转录系统。
Nucleic Acids Res. 1988 Nov 25;16(22):10469-76. doi: 10.1093/nar/16.22.10469.
2
Activation of vitellogenin gene transcription is a direct response to estrogen in Xenopus laevis liver.在非洲爪蟾肝脏中,卵黄蛋白原基因转录的激活是对雌激素的直接反应。
Nucleic Acids Res. 1982 Dec 20;10(24):8273-84. doi: 10.1093/nar/10.24.8273.
3
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Proc Natl Acad Sci U S A. 1985 Feb;82(4):988-91. doi: 10.1073/pnas.82.4.988.
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Estrogen receptor level determines sex-specific in vitro transcription from the Xenopus vitellogenin promoter.雌激素受体水平决定了非洲爪蟾卵黄蛋白原启动子的性别特异性体外转录。
Proc Natl Acad Sci U S A. 1990 Oct;87(20):7878-82. doi: 10.1073/pnas.87.20.7878.
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The role of estrogen receptor in Xenopus laevis vitellogenin gene expression.雌激素受体在非洲爪蟾卵黄蛋白原基因表达中的作用。
Am J Physiol. 1982 Jul;243(1):C1-6. doi: 10.1152/ajpendo.1982.243.1.E1.
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Simultaneous analysis of conformation and transcription of A and B groups of vitellogenin genes in male and female Xenopus during primary and secondary activation by estrogen.雌激素初次和二次激活过程中非洲爪蟾雌雄个体中卵黄蛋白原基因A组和B组的构象与转录的同步分析
Nucleic Acids Res. 1983 Feb 25;11(4):1151-66. doi: 10.1093/nar/11.4.1151.
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Activation of chromosomal vitellogenin genes in Xenopus oocytes by pure estrogen receptor and independent activation of albumin genes.纯雌激素受体激活非洲爪蟾卵母细胞中的染色体卵黄蛋白原基因以及白蛋白基因的独立激活。
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Expression and translocation of cloned human estrogen receptor in the Xenopus oocyte does not induce expression of the endogenous oocyte vitellogenin genes.克隆的人雌激素受体在非洲爪蟾卵母细胞中的表达及转位不会诱导内源性卵母细胞卵黄生成素基因的表达。
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A liver protein fraction regulating hormone-dependent in vitro transcription from the vitellogenin genes induces their expression in Xenopus oocytes.一种调节卵黄蛋白原基因激素依赖性体外转录的肝脏蛋白质组分可诱导其在非洲爪蟾卵母细胞中的表达。
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The human estrogen receptor can regulate exogenous but not endogenous vitellogenin gene promoters in a Xenopus cell line.人类雌激素受体可在非洲爪蟾细胞系中调控外源性而非内源性卵黄蛋白原基因启动子。
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Activation of chromosomal vitellogenin genes in Xenopus oocytes by pure estrogen receptor and independent activation of albumin genes.纯雌激素受体激活非洲爪蟾卵母细胞中的染色体卵黄蛋白原基因以及白蛋白基因的独立激活。
Mol Cell Biol. 1990 Dec;10(12):6674-82. doi: 10.1128/mcb.10.12.6674-6682.1990.
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Specific transcription and reinitiation of 2,4-D-induced genes in tobacco nuclei.

本文引用的文献

1
Vitellogenin gene expression in male Xenopus hepatocytes during primary and secondary stimulation with estrogen in cell cultures.在细胞培养中,雌激素对非洲爪蟾雄性肝细胞进行初次和二次刺激时卵黄蛋白原基因的表达
Cell. 1981 Mar;23(3):741-6. doi: 10.1016/0092-8674(81)90437-2.
2
Scattering of repetitive DNA sequences in the albumin and vitellogenin gene loci of Xenopus laevis.非洲爪蟾白蛋白和卵黄蛋白原基因位点中重复DNA序列的散布
Nucleic Acids Res. 1983 Nov 25;11(22):7701-16. doi: 10.1093/nar/11.22.7701.
3
Partial purification of estradiol receptor from Xenopus laevis liver and levels of receptor in relation to estradiol concentration.
烟草细胞核中2,4-D诱导基因的特异性转录与再起始
Plant Mol Biol. 1992 Jan;18(1):109-17. doi: 10.1007/BF00018461.
从非洲爪蟾肝脏中对雌二醇受体进行部分纯化以及受体水平与雌二醇浓度的关系。
EMBO J. 1983;2(6):973-7. doi: 10.1002/j.1460-2075.1983.tb01530.x.
4
Injection of partially purified estrogen receptor protein from Xenopus liver nuclei into oocytes activates the silent vitellogenin locus.将非洲爪蟾肝细胞核中部分纯化的雌激素受体蛋白注射到卵母细胞中,可激活沉默的卵黄蛋白原基因座。
Proc Natl Acad Sci U S A. 1984 Sep;81(18):5777-81. doi: 10.1073/pnas.81.18.5777.
5
Sequence homologies in the region preceding the transcription initiation site of the liver estrogen-responsive vitellogenin and apo-VLDLII genes.肝脏雌激素反应性卵黄蛋白原和载脂蛋白-VLDLII基因转录起始位点之前区域的序列同源性。
Nucleic Acids Res. 1984 Nov 26;12(22):8611-26. doi: 10.1093/nar/12.22.8611.
6
Evolution of vitellogenin genes: comparative analysis of the nucleotide sequences downstream of the transcription initiation site of four Xenopus laevis and one chicken gene.卵黄蛋白原基因的进化:对四种非洲爪蟾和一种鸡基因转录起始位点下游核苷酸序列的比较分析。
Nucleic Acids Res. 1984 Nov 26;12(22):8595-609. doi: 10.1093/nar/12.22.8595.
7
A general method for preparing intact nuclear DNA.一种制备完整核DNA的通用方法。
EMBO J. 1984 Aug;3(8):1837-42. doi: 10.1002/j.1460-2075.1984.tb02056.x.
8
Entrapment of animal cells for production of monoclonal antibodies and other biomolecules.用于生产单克隆抗体和其他生物分子的动物细胞截留。
Nature. 1983 Apr 14;302(5909):629-30. doi: 10.1038/302629a0.
9
Isolation and characterisation of the Xenopus laevis albumin genes: loss of 74K albumin gene sequences by library amplification.非洲爪蟾白蛋白基因的分离与鉴定:文库扩增导致74K白蛋白基因序列丢失。
Nucleic Acids Res. 1982 May 11;10(9):2791-807. doi: 10.1093/nar/10.9.2791.
10
Quantitation of estrogen effect on Xenopus laevis albumin mRNA levels by hybridization to cloned albumin cDNA.通过与克隆的白蛋白互补DNA杂交来定量雌激素对非洲爪蟾白蛋白信使核糖核酸水平的影响。
J Biol Chem. 1982 Jul 25;257(14):8496-501.