Department of Organic Biomaterials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, 101-0062, Japan.
Denka Innovation Center, Denka Co., Ltd., Machida, Tokyo, 194-8560, Japan.
Macromol Biosci. 2020 Apr;20(4):e1900424. doi: 10.1002/mabi.201900424. Epub 2020 Feb 14.
Polyrotaxanes, consisting of poly(ethylene glycol) and α-cyclodextrins, are mechanically interlocked supermolecules. The structure allows α-cyclodextrins to move along the polymer, referred to as molecular mobility. Here, polyrotaxane-based triblock copolymers, composed of polyrotaxanes with different degrees of methylation and poly(benzyl methacrylate) at both terminals, are coated on culture surfaces to fabricate dynamic biointerfaces for myocyte differentiation. The molecular mobility increases with the degree of methylation and the contact angle hysteresis of water droplets and air bubbles. When the mouse myoblast cell line C2C12 is cultured on methylated polyrotaxane surfaces, the expression levels of myogenesis-related genes, myogenin (Myog) and myosin heavy chain (Myhc) are altered by the degree of methylation. Polyrotaxane surfaces with intermediate degrees of methylation promote the highest expression levels among all the surfaces. The polyrotaxane surface provides an appropriate environment for myocyte differentiation by accurately adjusting the degrees of methylation.
聚轮烷是由聚乙二醇和α-环糊精组成的机械互锁超分子。这种结构允许α-环糊精在聚合物上移动，这被称为分子迁移性。在这里，基于聚轮烷的三嵌段共聚物由具有不同甲基化程度的聚轮烷和两端的聚（苯甲基甲基丙烯酸酯）组成，被涂覆在培养表面上，以制造用于肌细胞分化的动态生物界面。分子迁移性随甲基化程度和水滴滴和气泡的接触角滞后增加。当小鼠成肌细胞系 C2C12 培养在甲基化聚轮烷表面上时，甲基化程度改变了与肌生成相关的基因肌球蛋白（Myog）和肌球蛋白重链（Myhc）的表达水平。在所有表面中，具有中等甲基化程度的聚轮烷表面促进了最高的表达水平。聚轮烷表面通过准确调整甲基化程度为肌细胞分化提供了适宜的环境。
