Sekiya-Aoyama Ruriko, Arisaka Yoshinori, Hakariya Masahiro, Masuda Hiroki, Iwata Takanori, Yoda Tetsuya, Yui Nobuhiko
Department of Organic Biomaterials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University (TMDU), 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan.
Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo, Tokyo 113-8549, Japan.
Biomater Sci. 2021 Feb 9;9(3):675-684. doi: 10.1039/d0bm01782e.
Polyrotaxanes are supramolecular assemblies consisting of cyclic molecules (e.g., α-cyclodextrins) and linear polymer chains (e.g., poly[ethylene glycol]), in which cyclic molecules can move along the polymer chain. Here, we examined the effect of functional groups introduced into the α-cyclodextrins of polyrotaxane on cell responses such as adhesion, proliferation, and differentiation. Polyrotaxane-based triblock copolymers modified with methyl (CH3, hydrophobic, and nonionic), hydroxy (OH, hydrophilic and nonionic), amino (NH2, cationic), and sulfo (SO3H, anionic) groups were coated on the surface of the culture plate to fabricate polyrotaxane surfaces with different surface chemistries. The chemical compositions of each surface were determined via time-of-flight secondary ion mass spectrometry and X-ray photoelectron spectroscopy. The contact angle hysteresis reflecting the molecular mobility and zeta potential of each polyrotaxane surface changed depending on the functional groups. When osteoblast and adipocyte differentiation was induced in human mesenchymal stem cells cultured on each polyrotaxane surface, the cells adhered to the SO3H-modified polyrotaxane surfaces exhibited osteoblast differentiation, whereas the cells adhered to the OH-, NH2-, and SO3H-modified polyrotaxane surfaces preferentially underwent adipocyte differentiation compared with those on the unmodified and CH3-modified polyrotaxane surfaces. Interestingly, the SO3H-modified polyrotaxane surfaces promoted both osteoblast and adipocyte differentiation. High molecular mobility and negative charge on the SO3H-modified polyrotaxane surfaces are expected to contribute to the facilitation of both osteoblast and adipocyte differentiation.
聚轮烷是由环状分子（如α-环糊精）和线性聚合物链（如聚乙二醇）组成的超分子聚集体，其中环状分子可沿聚合物链移动。在此，我们研究了引入聚轮烷α-环糊精中的官能团对细胞反应（如黏附、增殖和分化）的影响。用甲基（CH3，疏水且非离子）、羟基（OH，亲水且非离子）、氨基（NH2，阳离子）和磺酸基（SO3H，阴离子）修饰的基于聚轮烷的三嵌段共聚物被涂覆在培养板表面，以制备具有不同表面化学性质的聚轮烷表面。通过飞行时间二次离子质谱和X射线光电子能谱确定每个表面的化学成分。反映每个聚轮烷表面分子流动性和zeta电位的接触角滞后随官能团而变化。当在每个聚轮烷表面培养的人间充质干细胞中诱导成骨细胞和脂肪细胞分化时，黏附在SO3H修饰的聚轮烷表面的细胞表现出成骨细胞分化，而与未修饰和CH3修饰的聚轮烷表面相比，黏附在OH-、NH2-和SO3H修饰的聚轮烷表面的细胞优先进行脂肪细胞分化。有趣的是，SO3H修饰的聚轮烷表面促进了成骨细胞和脂肪细胞的分化。预计SO3H修饰的聚轮烷表面上的高分子流动性和负电荷有助于促进成骨细胞和脂肪细胞的分化。
