Institute of Biomedical Informatics, Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, 325035, China.
Wenzhou Vocational College of Science and Technology, Wenzhou, 325000, China.
Comp Immunol Microbiol Infect Dis. 2020 Apr;69:101427. doi: 10.1016/j.cimid.2020.101427. Epub 2020 Jan 26.
The objective of this study was to investigate the molecular characteristics and horizontal transfer of florfenicol resistance gene-related sequences in Proteus strains isolated from animals. A total of six Proteus strains isolated from three farms between 2015 and 2016 were screened by polymerase chain reaction (PCR) for known florfenicol resistance genes. Proteus cibarius G11, isolated from the fecal material of a goose, was found to harbor both cfr and floR genes. Whole genome sequencing revealed that the strain harbored two copies of the floR gene: one was located on the chromosome and the other was located on a plasmid named pG11-152. Two floR-containing fragments 4028 bp in length were identical and showed transposon-like structures. The cfr gene was found on a plasmid named pG11-51 and flanked by a pair of IS26s. Thus, mobile genetic elements played an important role in floR replication and horizontal resistance gene transfer. Therefore, increasing attention should be paid to monitoring the spread of resistance genes and resistance in real time.
本研究旨在探讨从动物中分离的变形杆菌属菌株中氟苯尼考耐药基因相关序列的分子特征和水平转移。通过聚合酶链反应（PCR）对 2015 年至 2016 年间从三个农场分离的六株变形杆菌属菌株进行了已知氟苯尼考耐药基因的筛选。从鹅粪便中分离的变形杆菌属菌株 G11 被发现同时携带 cfr 和 floR 基因。全基因组测序显示，该菌株携带两个 floR 基因：一个位于染色体上，另一个位于一个名为 pG11-152 的质粒上。两个含有 floR 的片段长度为 4028bp，完全相同，表现出转座子样结构。cfr 基因位于一个名为 pG11-51 的质粒上，两侧是一对 IS26s。因此，移动遗传元件在 floR 复制和水平抗性基因转移中发挥了重要作用。因此，应更加关注实时监测耐药基因和抗性的传播。
