Department of Chemistry, Zhejiang University, Hangzhou, 310028, China; School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, 637457, Singapore.
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, 637457, Singapore.
Biomaterials. 2020 Apr;238:119847. doi: 10.1016/j.biomaterials.2020.119847. Epub 2020 Feb 7.
Functional proteins are essential for the regulation of cellular behaviors and have found growing therapeutic uses. However, low bioavailability of active proteins to their intracellular targets has been a long-standing challenge to achieve their full potential for cell reprogramming and disease treatment. Here we report mesoporous polydopamine (mPDA) with a built-in plasmonic nanoparticle core as a multifunctional protein delivery system. The mPDA with a unique combination of large surface area, metal-chelating property, and broad-spectrum photothermal transduction allows efficient loading and near-infrared light-triggered release of functional proteins, while the plasmonic core serves as a photostable tracer and fluorescence quencher, collectively leading to real-time monitoring and active cytosolic release of model proteins. In particular, controlled delivery of cytotoxic ribonuclease A has shown excellent performance in invivo cancer therapy. The possibility of coating mPDA on a broad range of functional cores, thanks to its universal adhesion, provides opportunities for developing tailored delivery carriers of biologics to overcome intrinsic biological barriers.
功能性蛋白质对于细胞行为的调节至关重要，并且在治疗方面的应用也越来越广泛。然而，活性蛋白质向其细胞内靶标传递的生物利用度低，一直是实现其在细胞重编程和疾病治疗方面的全部潜力的长期挑战。在这里，我们报告了具有内置等离子体纳米颗粒核的介孔聚多巴胺（mPDA）作为一种多功能蛋白质递送系统。mPDA 具有独特的大表面积、金属螯合特性和广谱光热转换能力的组合，允许高效负载和近红外光触发释放功能蛋白，而等离子体核则作为光稳定示踪剂和荧光猝灭剂，共同实现模型蛋白的实时监测和主动细胞质释放。特别是，细胞毒性核糖核酸酶 A 的控制释放在体内癌症治疗中表现出优异的性能。由于其普遍的粘附性，mPDA 可以在广泛的功能核心上进行涂层，这为开发针对生物制品的定制递送载体以克服内在的生物学障碍提供了机会。
